Gan Yu, Fleming Christine P
Department of Electrical Engineering, Columbia University, 500 West 120th Street, New York, New York, USA.
Biomed Opt Express. 2013 Sep 13;4(10):2150-65. doi: 10.1364/BOE.4.002150. eCollection 2013.
Abnormal changes in orientation of myofibers are associated with various cardiac diseases such as arrhythmia, irregular contraction, and cardiomyopathy. To extract fiber information, we present a method of quantifying fiber orientation and reconstructing three-dimensional tractography of myofibers using optical coherence tomography (OCT). A gradient based algorithm was developed to quantify fiber orientation in three dimensions and particle filtering technique was employed to track myofibers. Prior to image processing, three-dimensional image data set were acquired from all cardiac chambers and ventricular septum of swine hearts using OCT system without optical clearing. The algorithm was validated through rotation test and comparison with manual measurements. The experimental results demonstrate that we are able to visualize three-dimensional fiber tractography in myocardium tissues.
肌纤维方向的异常变化与各种心脏疾病相关，如心律失常、不规则收缩和心肌病。为了提取纤维信息，我们提出了一种使用光学相干断层扫描（OCT）量化纤维方向并重建肌纤维三维束状图的方法。开发了一种基于梯度的算法来量化三维纤维方向，并采用粒子滤波技术来跟踪肌纤维。在图像处理之前，使用未经光学清除的OCT系统从猪心脏的所有心腔和室间隔获取三维图像数据集。该算法通过旋转测试和与手动测量的比较进行了验证。实验结果表明，我们能够在心肌组织中可视化三维纤维束状图。
