小鼠细胞黏附分子埃-钙黏蛋白的分子克隆：互补DNA含有一个与B1相关的序列。
Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.
作者信息
Schuh R, Vestweber D, Riede I, Ringwald M, Rosenberg U B, Jäckle H, Kemler R
出版信息
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1364-8. doi: 10.1073/pnas.83.5.1364.
A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome.
通过免疫技术从表达载体λgt11中的cDNA文库中分离出一个包含细胞粘附分子桥粒芯糖蛋白编码序列的克隆（F20）。β-半乳糖苷酶-桥粒芯糖蛋白融合蛋白用于亲和纯化抗桥粒芯糖蛋白抗体。亲和纯化的抗体可识别胚胎癌细胞裂解物中的桥粒芯糖蛋白，并与胚胎癌细胞的细胞表面发生反应。1.8千碱基的cDNA插入片段与仅在表达桥粒芯糖蛋白的细胞中发现的单一4.3千碱基聚腺苷酸加尾RNA物种杂交。克隆的桥粒芯糖蛋白cDNA的部分非翻译3'序列与小鼠基因组的散布B1重复序列同源。
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