Muranaka M, Suzuki S, Koizumi K, Takafuji S, Miyamoto T, Ikemori R, Tokiwa H
J Allergy Clin Immunol. 1986 Apr;77(4):616-23. doi: 10.1016/0091-6749(86)90355-6.
The prevalence rate of allergic rhinitis caused by pollen has strikingly increased in Japan in the last three decades. The number of diesel cars in use has also rapidly increased in the country. This fact urged us to study the effects of particulates emitted from diesel cars on the production of IgE antibody. The primary IgE antibody responses in mice immunized with intraperitoneal injection of ovalbumin (OA) mixed with diesel-exhaust particulates (DEP) were higher than those in the animals immunized with OA alone. This effect of DEP on the production of IgE antibody in mice was also demonstrated when mice were immunized with repeated injections of dinitrophenylated-OA. In addition, persistent IgE-antibody response to major allergen of Japanese cedar pollen (JCPA), a most common pollen causing allergic rhinitis in Japan, was observed in mice immunized with JCPA mixed with DEP but not in the animals immunized with JCPA alone. The results do indicate that the adjuvant activity of DEP can not be excluded as a possible cause of the associated change in the number of diesel cars and allergic rhinitis caused by pollen in Japan.
在过去三十年中，日本由花粉引起的过敏性鼻炎患病率显著上升。该国使用的柴油车数量也迅速增加。这一事实促使我们研究柴油车排放的颗粒物对IgE抗体产生的影响。用腹腔注射与柴油废气颗粒物（DEP）混合的卵清蛋白（OA）免疫的小鼠，其主要IgE抗体反应高于仅用OA免疫的动物。当用二硝基苯基化-OA重复注射免疫小鼠时，也证明了DEP对小鼠IgE抗体产生的这种影响。此外，在用与DEP混合的日本柳杉花粉（JCPA，日本最常见的引起过敏性鼻炎的花粉）免疫的小鼠中观察到对JCPA主要过敏原的持续IgE抗体反应，而在仅用JCPA免疫的动物中未观察到。结果确实表明，不能排除DEP的佐剂活性可能是日本柴油车数量变化与花粉引起的过敏性鼻炎相关的一个原因。
