Takafuji S, Suzuki S, Koizumi K, Tadokoro K, Miyamoto T, Ikemori R, Muranaka M
J Allergy Clin Immunol. 1987 Apr;79(4):639-45. doi: 10.1016/s0091-6749(87)80161-6.
Our previous study indicated that the IgE antibody responses in mice immunized with intraperitoneal injection of the antigens mixed with diesel-exhaust particulates (DEP) were higher than those in the animals immunized with the antigens alone. We examined the adjuvant activity of DEP inoculated by the intranasal route, i.e., the natural entrance of DEP. In 3-week interval immunization, the IgE antibody responses in mice immunized with intranasal inoculation of ovalbumin (OA) mixed with DEP were higher than responses in the animals immunized with OA alone. DEP had an adjuvant activity for anti-OA IgE antibody production, even in a small dose such as 1 micrograms administered with a 3-week interval. Also in 1-week interval immunization, the enhancing effect of DEP on anti-OA IgE antibody production was demonstrated when mice were immunized with intranasal inoculation of OA and DEP. The possibility cannot be excluded that DEP, which are kept buoyant in the environmental atmosphere of urban districts, may exert an adjuvant activity for IgE antibody production after being inhaled into the human body and have some relation to the mechanism of the outbreak of allergic rhinitis caused by pollens in Japan.
我们之前的研究表明，腹腔注射与柴油废气颗粒（DEP）混合的抗原免疫的小鼠中，IgE抗体反应高于仅用抗原免疫的动物。我们研究了通过鼻内途径接种的DEP的佐剂活性，即DEP的自然进入途径。在间隔3周的免疫中，鼻内接种与DEP混合的卵清蛋白（OA）免疫的小鼠中，IgE抗体反应高于仅用OA免疫的动物。即使以1微克这样的小剂量、间隔3周给药，DEP对抗OA IgE抗体产生也具有佐剂活性。同样在间隔1周的免疫中，当小鼠鼻内接种OA和DEP时，也证明了DEP对抗OA IgE抗体产生的增强作用。不能排除在市区环境大气中保持悬浮状态的DEP，在被人体吸入后可能对IgE抗体产生发挥佐剂活性，并与日本由花粉引起的过敏性鼻炎的发病机制有某种关系。
