Chan-Palay V, Yasargil G
Anat Embryol (Berl). 1986;174(1):27-33. doi: 10.1007/BF00318333.
NPY-containing neuronal structures in the cerebral cortex of surgical tissue samples were compared to those in postmortem material by immunocytochemical methods. However, the quality of preservation of individual neurons and axonal and dendritic plexuses in the neuropil is unusually fine in the surgical specimens. This result is most likely attributable to the excellent fixation that can be regularly achieved by rapid and careful handling of tissue during and after surgical removal. The tissue is suitable for both light and electron microscopy, and the superior preservation also leads to intense, reliable antibody reactions. Postmortem tissue samples can provide good specimens for immunocytochemistry when properly handled as previously described. However the postmortem delays prior to fixation disrupt neuronal integrity in the immunostained structures. Nevertheless, postmortem material from carefully studied subjects of neurological diseases compared with age matched controls can provide valuable information.
通过免疫细胞化学方法，将手术组织样本大脑皮质中含神经肽Y（NPY）的神经元结构与尸检材料中的进行了比较。然而，手术标本中单个神经元以及神经毡中轴突和树突丛的保存质量异常良好。这一结果很可能归因于在手术切除过程中和切除后通过快速、仔细地处理组织能够常规实现的极佳固定效果。该组织适用于光学显微镜和电子显微镜观察，而且这种优越的保存效果还能带来强烈、可靠的抗体反应。如前所述，尸检组织样本经过妥善处理后可为免疫细胞化学提供良好的标本。然而，固定前的尸检延迟会破坏免疫染色结构中的神经元完整性。尽管如此，来自经过仔细研究的神经疾病患者的尸检材料与年龄匹配的对照组相比，仍能提供有价值的信息。
