Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM U964, Université de Strasbourg, F-67404 Illkirch, France;
Genes Dev. 2013 Nov 15;27(22):2427-32. doi: 10.1101/gad.224550.113.
Mammalian development begins with fertilization of an oocyte by the sperm followed by genome-wide epigenetic reprogramming. This involves de novo establishment of chromatin domains, including the formation of pericentric heterochromatin. We dissected the spatiotemporal kinetics of the first acquisition of heterochromatic signatures of pericentromeric chromatin and found that the heterochromatic marks follow a temporal order that depends on a specific nuclear localization. We addressed whether nuclear localization of pericentric chromatin is required for silencing by tethering it to the nuclear periphery and show that this results in defective silencing and impaired development. Our results indicate that reprogramming of pericentromeric heterochromatin is functionally linked to its nuclear localization.
哺乳动物的发育始于卵子被精子受精，随后进行全基因组的表观遗传重编程。这涉及到染色质域的从头建立，包括着丝粒异染色质的形成。我们剖析了第一个获得着丝粒周围染色质异染色质特征的时空动力学，发现异染色质标记遵循一个时间顺序，该顺序取决于特定的核定位。我们研究了着丝粒周围染色质的核定位是否是沉默所必需的，通过将其固定在核周来实现，并表明这导致沉默缺陷和发育受损。我们的结果表明，着丝粒异染色质的重编程与其核定位在功能上是相关的。
