Department of Plant Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, 260, Chiba, Japan.
Plant Cell Rep. 1989 Mar;7(8):607-10. doi: 10.1007/BF00272040.
Tobacco shooty or rooty teratomas and hairy roots were induced by Agrobacterium tumefaciens (pGV 3845), A. tumefaciens (pGV 3304) and A. rhizogenes (pRi 8196), respectively. The tobacco alkaloids, nicotine, nornicotine and anatabine, were produced in hairy roots and in rooty teratomas but not in shooty teratomas. However, the shooty teratomas have the ability to accumulate the alkaloids and to biotransform nicotine to nornicotine. These were established by co-culture experiments incubating hairy roots and shooty teratomas in a same dish and by biotransformation experiments with shooty teratomas.
发根农杆菌(pGV3845)、根癌农杆菌(pGV3304)和发根农杆菌(pRi8196)分别诱导烟草丛生芽和根性畸胎瘤及毛状根的形成。在毛状根和根性畸胎瘤中产生烟草生物碱，尼古丁、降烟碱和新烟碱，但在丛生芽中没有产生。然而，丛生芽有积累生物碱和将尼古丁生物转化为降烟碱的能力。这是通过在同一个培养皿中培养毛状根和丛生芽的共培养实验以及通过丛生芽的生物转化实验来确定的。
