Unit for Plant Cell and Molecular Biology and Department of Botany, University of Delhi, 110007, Delhi, India.
Plant Cell Rep. 1985 May;4(3):115-7. doi: 10.1007/BF00571293.
Organised structures develop on a white and compact callus initiated from small segments of immature inflorescences ofEchinochloa colonum cultured on Murashige and Skoog's (MS) medium supplemented with 5.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 10% coconut milk. These develop into plantlets upon subculture onto MS medium containing 0 or 0.2 mg/l 2,4-D. Twelve out of 17 plantlets regenerated grew well on transfer to soil and eleven plants produced seeds.
在添加了 5.0 mg/L 2,4-二氯苯氧乙酸（2,4-D）和 10%椰子乳的 Murashige 和 Skoog（MS）培养基上培养的成熟花序小片段，诱导形成白色紧凑的愈伤组织，在此基础上可发育出组织有序的结构。将这些愈伤组织继代到含有 0 或 0.2 mg/L 2,4-D 的 MS 培养基上，可进一步发育成小植株。在转移到土壤上后，17 株再生小植株中有 12 株生长良好，其中 11 株植物产生了种子。
