Dorniani Dena, Hussein Mohd Zobir Bin, Kura Aminu Umar, Fakurazi Sharida, Shaari Abdul Halim, Ahmad Zalinah
Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Selangor 43400, Malaysia.
Int J Mol Sci. 2013 Dec 3;14(12):23639-53. doi: 10.3390/ijms141223639.
The preparation of magnetic nanoparticles coated with chitosan-prindopril erbumine was accomplished and confirmed by X-ray diffraction, TEM, magnetic measurements, thermal analysis and infrared spectroscopic studies. X-ray diffraction and TEM results demonstrated that the magnetic nanoparticles were pure iron oxide phase, having a spherical shape with a mean diameter of 6 nm, compared to 15 nm after coating with chitosan-prindopril erbumine (FCPE). Fourier transform infrared spectroscopy study shows that the coating of iron oxide nanoparticles takes place due to the presence of some bands that were emerging after the coating process, which belong to the prindopril erbumine (PE). The thermal stability of the PE in an FCPE nanocomposite was remarkably enhanced. The release study showed that around 89% of PE could be released within about 93 hours by a phosphate buffer solution at pH 7.4, which was found to be of sustained manner governed by first order kinetic. Compared to the control (untreated), cell viability study in 3T3 cells at 72 h post exposure to both the nanoparticles and the pure drug was found to be sustained above 80% using different doses.
壳聚糖-培哚普利叔丁胺盐包覆的磁性纳米颗粒的制备通过X射线衍射、透射电子显微镜（TEM）、磁性测量、热分析和红外光谱研究得以完成并得到证实。X射线衍射和TEM结果表明，磁性纳米颗粒为纯氧化铁相，呈球形，平均直径为6nm，而壳聚糖-培哚普利叔丁胺盐（FCPE）包覆后平均直径为15nm。傅里叶变换红外光谱研究表明，氧化铁纳米颗粒的包覆是由于包覆过程后出现了一些属于培哚普利叔丁胺盐（PE）的谱带。FCPE纳米复合材料中PE的热稳定性显著提高。释放研究表明，在pH 7.4的磷酸盐缓冲溶液中，约89%的PE可在约93小时内释放，发现其释放方式为一级动力学控制的持续释放。与对照组（未处理）相比，在暴露于纳米颗粒和纯药物72小时后，使用不同剂量在3T3细胞中的细胞活力研究发现细胞活力维持在80%以上。
