Asirvatham Raju, Christina Arockiasamy Josphin Maria, Murali Anita
Department of Pharmacology, Shri Rawatpura Sarkar Institute of Pharmacy, Datia, Mathya Pradesh, India.
Adv Pharm Bull. 2013;3(1):115-20. doi: 10.5681/apb.2013.019. Epub 2013 Feb 7.
The aim of present in vitro studies was performed to examine the antioxidant and anticancer activities of ethanol and aqueous extracts of Drosera indica L.
Different concentrations (5 - 640mcg/ml) of the ethanol (EEDI) and aqueous (AEDI) extracts of D.indica L were used in various antioxidant assay methods such as hydroxyl radicals, DPPH, super oxide radical scavenging activity, chelating ability of ferrous ion, nitric oxide radical inhibition, ABTS and reducing power. Ascorbic acid (AA) was used as the standard antioxidant for the free radical scavenging assays. Dalton's Ascitic Lymphoma (DAL) and Ehrlich Ascitic Carcinoma (EAC) cell lines were used as the in vitro cancer models for the tryphan blue dye and LDH leakage assays, where 5 to 250mcg /ml of both EEDI and AEDI were tested.
EEDI showed antioxidant activities with the minimum IC50 values of 34.8±0.43 mcg/ml in scavenging of hydroxyl radical and moreover AEDI showed minimum IC50 values of 94.51±0.84 mcg/ml in Fe(2+)chelating assay. EEDI on the reducing power assay and ABTS showed higher IC50 than standard AA. IC50 values of AEDI on Fe(2+) chelating assay and super oxide radical assay was lesser than IC50 value of AA. Both extracts at 250mcg/ml dose showed remarkable increase in the percentage of dead cancer cells (90% by EEDI and 86% by AEDI in DAL model and 89% by EEDI and 80% by AEDI in EAC model).
It is concluded from this study that D.indica L exhibited excellent antioxidant activity against the different in vitro antioxidant models and anticancer activity against the two different cell lines tested.
进行本体外研究的目的是检测印度茅膏菜乙醇提取物和水提取物的抗氧化及抗癌活性。
印度茅膏菜的乙醇提取物（EEDI）和水提取物（AEDI）的不同浓度（5 - 640微克/毫升）被用于各种抗氧化检测方法，如羟基自由基、DPPH、超氧阴离子自由基清除活性、亚铁离子螯合能力、一氧化氮自由基抑制、ABTS和还原能力。抗坏血酸（AA）用作自由基清除检测的标准抗氧化剂。道尔顿腹水淋巴瘤（DAL）和艾氏腹水癌（EAC）细胞系用作体外癌症模型进行台盼蓝染料和乳酸脱氢酶泄漏检测，其中对5至250微克/毫升的EEDI和AEDI都进行了测试。
EEDI在清除羟基自由基方面表现出抗氧化活性，最低IC50值为34.8±0.43微克/毫升，此外，AEDI在Fe(2+)螯合检测中显示最低IC50值为94.51±0.84微克/毫升。EEDI在还原能力检测和ABTS检测中的IC50值高于标准AA。AEDI在Fe(2+)螯合检测和超氧阴离子自由基检测中的IC50值小于AA的IC50值。两种提取物在250微克/毫升剂量下均显示死癌细胞百分比显著增加（在DAL模型中，EEDI为90%，AEDI为86%；在EAC模型中，EEDI为89%，AEDI为80%）。
本研究得出结论，印度茅膏菜对不同的体外抗氧化模型表现出优异的抗氧化活性，对所测试的两种不同细胞系具有抗癌活性。
