Lopes Helena B, Santos Thiago de S, de Oliveira Fabiola S, Freitas Gileade P, de Almeida Adriana Lg, Gimenes Rossano, Rosa Adalberto L, Beloti Marcio M
Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Institute of Physics and Chemistry, Federal University of Itajubá, Itajubá, MG, Brazil.
J Biomater Appl. 2014 Jul;29(1):104-12. doi: 10.1177/0885328213515735. Epub 2013 Dec 6.
In this study, we evaluated the effect of poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane on in vivo bone formation. Rat calvarial bone defects were implanted with P(VDF-TrFE)/BT and polytetrafluoroethylene (PTFE) membranes, and at 4 and 8 weeks, histomorphometric and gene expression analyses were performed. A higher amount of bone formation was noticed on P(VDF-TrFE)/BT compared with PTFE. The gene expression of RUNX2, bone sialoprotein, osteocalcin, receptor activator of nuclear factor-kappa B ligand, and osteoprotegerin indicates that P(VDF-TrFE)/BT favored the osteoblast differentiation compared with PTFE. These results evidenced the benefits of using P(VDF-TrFE)/BT to promote new bone formation, which may represent a promising alternative to be employed in guided bone regeneration.
在本研究中，我们评估了聚（偏二氟乙烯-三氟乙烯）/钛酸钡（P(VDF-TrFE)/BT）膜对体内骨形成的影响。将P(VDF-TrFE)/BT膜和聚四氟乙烯（PTFE）膜植入大鼠颅骨骨缺损处，并在4周和8周时进行组织形态计量学和基因表达分析。与PTFE相比，在P(VDF-TrFE)/BT膜上观察到更多的骨形成。RUNX2、骨唾液蛋白、骨钙素、核因子κB受体激活剂配体和骨保护素的基因表达表明，与PTFE相比，P(VDF-TrFE)/BT更有利于成骨细胞分化。这些结果证明了使用P(VDF-TrFE)/BT促进新骨形成的益处，这可能是引导骨再生中一种有前景的替代方法。
