Liu Yuanyuan, Luo Yi, Wu Jing, Wang Yinsong, Yang Xiaoying, Yang Rui, Wang Baiqi, Yang Jinrong, Zhang Ning
Research Center of Basic Medical Science & School of Pharmacy, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin 300070, People's Republic of China.
Sci Rep. 2013 Dec 11;3:3469. doi: 10.1038/srep03469.
Graphene oxide (GO) has attracted enormous interests due to its extraordinary properties. Recent studies have confirmed the cytotoxicity of GO, we further investigate its mutagenic potential in this study. The results showed that GO interfered with DNA replication and induced mutagenesis at molecular level. GO treatments at concentrations of 10 and 100 μg/mL altered gene expression patterns at cellular level, and 101 differentially expressed genes mediated DNA-damage control, cell apoptosis, cell cycle, and metabolism. Intravenous injection of GO at 4 mg/kg for 5 consecutive days clearly induced formation of micronucleated polychromic erythrocytes in mice, and its mutagenesis potential appeared to be comparable to cyclophosphamide, a classic mutagen. In conclusion, GO can induce mutagenesis both in vitro and in vivo, thus extra consideration is required for its biomedical applications.
氧化石墨烯（GO）因其非凡的特性而引起了极大的关注。最近的研究证实了GO的细胞毒性，在本研究中我们进一步探究了其致突变潜力。结果表明，GO在分子水平上干扰DNA复制并诱导突变。10和100μg/mL浓度的GO处理在细胞水平上改变了基因表达模式，101个差异表达基因介导了DNA损伤控制、细胞凋亡、细胞周期和代谢。连续5天以4mg/kg的剂量静脉注射GO明显诱导了小鼠体内微核多染红细胞的形成，其致突变潜力似乎与经典诱变剂环磷酰胺相当。总之，GO在体外和体内均可诱导突变，因此其生物医学应用需要格外谨慎。
