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细胞角蛋白、波形蛋白和神经丝蛋白在人脉络丛中的共存。神经上皮组织中间丝的免疫组织化学研究。

Coexistence of cytokeratin, vimentin and neurofilament protein in human choroid plexus. An immunohistochemical study of intermediate filaments in neuroepithelial tissues.

作者信息

Kasper M, Goertchen R, Stosiek P, Perry G, Karsten U

出版信息

Virchows Arch A Pathol Anat Histopathol. 1986;410(3):173-7. doi: 10.1007/BF00710822.

DOI:10.1007/BF00710822
PMID:2432723
Abstract

The expression of intermediate filament proteins in human brain ependyma and choroid plexus epithelium has been studied by immunohistochemistry using a panel of monoclonal antibodies directed against all five classes of intermediate filaments. Ependymal cells express GFAP and vimentin filaments, whereas plexus epithelium simultaneously contains neurofilaments, cytokeratins and vimentin, a phenomenon not previously observed in normal cells in vivo. By means of specific antibodies we were able to establish that cytokeratins 8 and 18 but not 19 are present in plexus epithelium.

摘要

利用一组针对所有五类中间丝的单克隆抗体,通过免疫组织化学方法研究了人脑海绵体室管膜和脉络丛上皮中中间丝蛋白的表达。室管膜细胞表达胶质纤维酸性蛋白(GFAP)和波形蛋白丝,而脉络丛上皮同时含有神经丝、细胞角蛋白和波形蛋白,这一现象在体内正常细胞中以前未被观察到。通过特异性抗体,我们能够确定脉络丛上皮中存在细胞角蛋白8和18,但不存在细胞角蛋白19。

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1
Coexistence of cytokeratin, vimentin and neurofilament protein in human choroid plexus. An immunohistochemical study of intermediate filaments in neuroepithelial tissues.细胞角蛋白、波形蛋白和神经丝蛋白在人脉络丛中的共存。神经上皮组织中间丝的免疫组织化学研究。
Virchows Arch A Pathol Anat Histopathol. 1986;410(3):173-7. doi: 10.1007/BF00710822.
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[Comparative immunohistochemical and lectin histochemical studies of ependymal cells and the epithelium of the choroid plexus].室管膜细胞与脉络丛上皮的比较免疫组织化学和凝集素组织化学研究
Acta Histochem. 1987;82(2):199-209.
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Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin.中间丝蛋白在人牙釉质器官中的分布:细胞角蛋白多肽和波形蛋白共表达的异常复杂模式。
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A Chronic Fatigue Syndrome - related proteome in human cerebrospinal fluid.人类脑脊液中与慢性疲劳综合征相关的蛋白质组。
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Intermediate filaments in the nervous system: implications in cancer.神经系统中的中间丝:对癌症的影响

本文引用的文献

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Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation.针对小鼠囊胚形成过程中滋养外胚层特异性标志物的单克隆抗体。
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Coexpression of cytokeratin and vimentin in Rathke's cysts of the human pituitary gland.细胞角蛋白和波形蛋白在人垂体Rathke囊肿中的共表达。
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10
Immunohistochemical demonstration of the co-expression of vimentin and cytokeratin(s) in the guinea pig cochlea.波形蛋白和细胞角蛋白在豚鼠耳蜗中共表达的免疫组织化学证明
Arch Otorhinolaryngol. 1987;244(1):66-8. doi: 10.1007/BF00453494.
单克隆抗体可在原位区分神经丝的磷酸化和非磷酸化形式。
Proc Natl Acad Sci U S A. 1983 Oct;80(19):6126-30. doi: 10.1073/pnas.80.19.6126.
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Monoclonal antibodies against antigens of the human mammary carcinoma cell line MCF-7.
Arch Geschwulstforsch. 1983;53(6):529-36.
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Human monoclonal IgM with autoantibody activity against intermediate filaments.具有抗中间丝自身抗体活性的人单克隆IgM
Proc Natl Acad Sci U S A. 1982 Jan;79(2):446-50. doi: 10.1073/pnas.79.2.446.
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Bodian's silver method stains neurofilament polypeptides.博迪恩银染法可对神经丝多肽进行染色。
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Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations.使用角蛋白单克隆抗体对人类上皮及其肿瘤进行分类:策略、应用及局限性
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Detection of cytokeratin(s) in epithelium of human plexus choroideus by monoclonal antibodies.
Acta Histochem. 1986;78(1):101-3. doi: 10.1016/S0065-1281(86)80016-2.
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Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.由电融合产生的杂交瘤克隆制备的单克隆抗细胞角蛋白抗体。
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