DST/NRF Centre of Excellence for Biomedical Tuberculosis Research and MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Stellenbosch, P.O. Box 19063, Francie van Zijl Drive, Tygerberg 7505, South Africa.
Mediators Inflamm. 2013;2013:981984. doi: 10.1155/2013/981984. Epub 2013 Nov 13.
The diagnosis of tuberculosis remains challenging in individuals with difficulty in providing good quality sputum samples such as children. Host biosignatures of inflammatory markers may be valuable in such cases, especially if they are based on more easily obtainable samples such as saliva. To explore the potential of saliva as an alternative sample in tuberculosis diagnostic/biomarker investigations, we evaluated the levels of 33 host markers in saliva samples from individuals presenting with pulmonary tuberculosis symptoms and compared them to those obtained in serum. Of the 38 individuals included in the study, tuberculosis disease was confirmed in 11 (28.9%) by sputum culture. In both the tuberculosis cases and noncases, the levels of most markers were above the minimum detectable limit in both sample types, but there was no consistent pattern regarding the ratio of markers in serum/saliva. Fractalkine, IL-17, IL-6, IL-9, MIP-1 β , CRP, VEGF, and IL-5 levels in saliva and IL-6, IL-2, SAP, and SAA levels in serum were significantly higher in tuberculosis patients (P < 0.05). These preliminary data indicate that there are significant differences in the levels of host markers expressed in saliva in comparison to those expressed in serum and that inflammatory markers in both sample types are potential diagnostic candidates for tuberculosis disease.
结核病的诊断在难以提供高质量痰样本的人群中仍然具有挑战性，如儿童。宿主炎症标志物的生物标志物在这种情况下可能很有价值，特别是如果它们基于更容易获得的样本，如唾液。为了探索唾液作为结核病诊断/生物标志物研究替代样本的潜力，我们评估了来自有肺结核症状的个体的唾液样本中 33 种宿主标志物的水平，并将其与血清中获得的水平进行了比较。在纳入研究的 38 名个体中，11 名（28.9%）通过痰培养确诊为结核病。在结核病病例和非病例中，大多数标志物在两种样本类型中的水平均高于最低可检测限度，但血清/唾液中标志物的比值没有一致的模式。唾液中的 fractalkine、IL-17、IL-6、IL-9、MIP-1β、CRP、VEGF 和 IL-5 水平以及血清中的 IL-6、IL-2、SAP 和 SAA 水平在结核病患者中显著升高（P<0.05）。这些初步数据表明，与血清相比，唾液中表达的宿主标志物水平存在显著差异，并且两种样本类型中的炎症标志物均是结核病疾病的潜在诊断候选物。
