Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland School of Dentistry, Baltimore, Maryland 21201, USA.
J Biomed Mater Res B Appl Biomater. 2013 Aug;101(6):929-38. doi: 10.1002/jbm.b.32898. Epub 2013 Mar 26.
Dental resins containing 12-methacryloyloxydodecylpyridinium bromide (MDPB) showed potent antibacterial functions. Recent studies developed antibacterial resins containing nanoparticles of silver (NAg). The objectives of this study were to develop an adhesive containing dual agents of MDPB and NAg for the first time and to investigate the combined effects of antibacterial adhesive and primer on biofilm viability, metabolic activity, lactic acid, dentin bond strength, and fibroblast cytotoxicity. MDPB and NAg were incorporated into Scotchbond Multi-Purpose (SBMP) adhesive "A" and primer "P". Five systems were tested: SBMP adhesive A; A + MDPB; A+NAg; A + MDPB + NAg; P + MDPB + NAg together with A + MDPB + NAg. Dental plaque microcosm biofilms were cultured using mixed saliva from 10 donors. Metabolic activity, colony-forming units, and lactic acid production of biofilms were investigated. Human fibroblast cytotoxicity of bonding agents was determined. MDPB + NAg in adhesive/primer did not compromise dentin bond strength (p > 0.1). MDPB or NAg alone in adhesive substantially reduced the biofilm activities. Dual agents MDPB + NAg in adhesive significantly reduced the biofilm viability compared with each agent alone (p < 0.05). The greatest inhibition of biofilms was achieved when both adhesive and primer contained MDPB + NAg. Fibroblast viability of groups with dual antibacterial agents was similar to control using culture medium without resin eluents (p > 0.1). In conclusion, this study showed for the first time that the antibacterial potency of MDPB adhesive could be substantially enhanced via NAg. Adding MDPB + NAg into both primer and adhesive achieved the strongest antibiofilm efficacy. The dual agent (MDPB + NAg) method could have wide applicability to other adhesives, sealants, cements, and composites to inhibit biofilms and caries.
含 12- 甲丙烯酰氧十二烷基溴化吡啶鎓（MDPB）的牙科树脂具有很强的抗菌功能。最近的研究开发了含有纳米银（NAg）的抗菌树脂。本研究的目的是首次开发含有 MDPB 和 NAg 双重剂的胶粘剂，并研究抗菌胶粘剂和底漆对生物膜活力、代谢活性、乳酸、牙本质粘结强度和成纤维细胞细胞毒性的综合影响。将 MDPB 和 NAg 掺入 Scotchbond Multi-Purpose (SBMP) 胶粘剂“A”和底漆“P”中。测试了五个系统：SBMP 胶粘剂 A；A + MDPB；A+NAg；A + MDPB + NAg；P + MDPB + NAg 与 A + MDPB + NAg 一起。使用来自 10 个供体的混合唾液培养牙菌斑微宇宙生物膜。研究了生物膜的代谢活性、菌落形成单位和乳酸产生。测定粘结剂对人成纤维细胞的细胞毒性。胶粘剂/底漆中的 MDPB + NAg 不会降低牙本质粘结强度（p > 0.1）。胶粘剂中单用 MDPB 或 NAg 可显著降低生物膜活性。与单独使用每种试剂相比，胶粘剂中的双重试剂 MDPB + NAg 可显著降低生物膜活力（p < 0.05）。当胶粘剂和底漆均含有 MDPB + NAg 时，对生物膜的抑制作用最大。含有双重抗菌剂的各组的成纤维细胞活力与不含树脂洗脱剂的培养基对照相似（p > 0.1）。总之，本研究首次表明，通过 NAg 可显著增强 MDPB 胶粘剂的抗菌效力。将 MDPB + NAg 添加到底漆和胶粘剂中可实现最强的抗生物膜效果。双重剂（MDPB + NAg）方法可能广泛适用于其他胶粘剂、密封剂、水泥和复合材料，以抑制生物膜和龋齿。
