Yamaguchi H, Morimatsu M, Hirai S, Takahashi K
Acta Neuropathol. 1987;72(3):214-7. doi: 10.1007/BF00691092.
Alzheimer's neurofibrillary tangles (ANT) in the hippocampal area were studied immunohistochemically using antisera against glial fibrillary acidic protein (GFAP) and S-100 protein in 48 patients with or without dementia between 52 and 92 years old. In 27 of the 38 brains that developed ANT in the hippocampal area, some ANT were immunostained with these antisera. Flame-shaped or globose-shaped immunostains were occasionally continuous with astroglial cell bodies and processes. They appeared particularly in the entorhinal cortex, subiculum and CA1. The ANT, immunostained with GFAP and S-100 antisera, apparently correspond to slightly eosinophilic tangles in H&E sections and to less argentophilic tangles in silver-impregnated sections in all of the 27 brains. ANT of another 11 brains were consistently negative with these antisera. The GFAP-positive eosinophilic tangles were encountered in the brains of older patients (P less than 0.01) and with more abundant formation of ANT (P less than 0.001). This alteration was present in all of the 20 brains with more than 100 ANT per section and none of the eight brains with less than 10 ANT. These findings suggest that in the last stages, ANT are penetrated by eosinophilic processes of astrocytes, and appear eosinophilic, and that the presence of GFAP-positive eosinophilic tangles indicates the abundant formation of ANT.
采用抗胶质纤维酸性蛋白（GFAP）和S-100蛋白的抗血清，对48例年龄在52至92岁之间、患有或未患痴呆症的患者海马区的阿尔茨海默病神经原纤维缠结（ANT）进行免疫组织化学研究。在海马区出现ANT的38个大脑中，有27个大脑的部分ANT被这些抗血清免疫染色。火焰状或球状免疫染色偶尔与星形胶质细胞体和突起相连。它们尤其出现在内嗅皮质、海马下托和CA1区。在所有27个大脑中，用GFAP和S-100抗血清免疫染色的ANT，在苏木精-伊红（H&E）切片中明显对应于轻度嗜酸性缠结，在银浸染切片中对应于嗜银性较低的缠结。另外11个大脑的ANT对这些抗血清始终呈阴性。GFAP阳性嗜酸性缠结在老年患者的大脑中出现（P<0.01），且ANT形成更丰富（P<0.001）。这种改变在每切片有超过100个ANT的所有20个大脑中均存在，而在每切片少于10个ANT的8个大脑中均未出现。这些发现表明，在最后阶段，ANT被星形胶质细胞的嗜酸性突起穿透，并呈现嗜酸性，并且GFAP阳性嗜酸性缠结的存在表明ANT形成丰富。
