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Ro60、Ro52和SS-B抗体检测新技术亮点

Highlights on novel technologies for the detection of antibodies to Ro60, Ro52, and SS-B.

作者信息

Infantino M, Bentow C, Seaman A, Benucci M, Atzeni F, Sarzi-Puttini P, Olivito B, Meacci F, Manfredi M, Mahler M

机构信息

Immunology and Allergology Laboratory Unit, Azienda Sanitaria di Firenze, San Giovanni di Dio Hospital, Florence, Italy.

Department of Research, INOVA Diagnostics Inc., 9900 Old Grove Road, San Diego, CA 92131-1638, USA.

出版信息

Clin Dev Immunol. 2013;2013:978202. doi: 10.1155/2013/978202. Epub 2013 Nov 27.

DOI:10.1155/2013/978202
PMID:24376466
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3860079/
Abstract

OBJECTIVE

We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B.

METHODS

The study included 241 samples, from patients suffering from systemic autoimmune diseases (n = 108) as well as disease controls (n = 133). All samples were tested for anti-Ro52, anti-Ro60, and anti-SS-B (La) antibodies on QUANTA Flash (INOVA Diagnostics, San Diego, USA) and BioPlex 2200 (Bio-Rad Laboratories Inc., Hercules, USA). Discrepant samples were tested by two independent methods: BlueDot/ANA and QUANTRIX Microarray (both D-tek, Belgium).

RESULTS

The overall qualitative agreements were 95.4% (95% confidence interval, CI 92.0-97.7%) for anti-Ro52, 98.8% (95% CI 96.4-99.7%) for anti-Ro60, and 91.7% (95% CI 87.5-94.9%) for anti-SS-B antibodies. There were 34 discrepant samples among all assays (20 anti-SS-B, 11 anti-Ro52, 3 anti-Ro60). 30/33 of retested samples (by D-tek dot blot) agreed with the QUANTA Flash results. Similar findings were obtained with QUANTRIX Microarray kit.

CONCLUSION

QUANTA Flash and BioPlex 2200 show good qualitative agreement. The clinical performances were similar for anti-Ro52 and anti-Ro60 autoantibodies while differences were observed for anti-SS-B (La) antibodies.

摘要

目的

我们旨在比较BIO-FLASH上的化学发光免疫分析（CIA，QUANTA Flash）与BioPlex 2200上的多重流式免疫分析（MFI）用于检测抗Ro60、Ro52和SS-B抗体。

方法

该研究纳入了241份样本，来自患有系统性自身免疫性疾病的患者（n = 108）以及疾病对照（n = 133）。所有样本均在QUANTA Flash（美国圣地亚哥的INOVA诊断公司）和BioPlex 2200（美国 Hercules的Bio-Rad实验室公司）上检测抗Ro52、抗Ro60和抗SS-B（La）抗体。对有差异的样本采用两种独立方法进行检测：BlueDot/ANA和QUANTRIX微阵列（均为比利时的D-tek公司）。

结果

抗Ro52的总体定性一致性为95.4%（95%置信区间，CI 92.0 - 97.7%），抗Ro60为98.8%（CI 96.4 - 99.7%），抗SS-B抗体为91.7%（CI 87.5 - 94.9%）。所有检测中共有34份有差异的样本（20份抗SS-B、11份抗Ro52、3份抗Ro60）。重新检测的样本（通过D-tek斑点印迹法）中有30/33与QUANTA Flash结果一致。使用QUANTRIX微阵列试剂盒也得到了类似结果。

结论

QUANTA Flash和BioPlex 2200显示出良好的定性一致性。抗Ro52和抗Ro60自身抗体的临床性能相似，而抗SS-B（La）抗体则观察到差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/66d508106da9/CDI2013-978202.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/9dee87a9e8d6/CDI2013-978202.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/ea58a7598822/CDI2013-978202.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/dcdbb05e1cc9/CDI2013-978202.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/66d508106da9/CDI2013-978202.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/9dee87a9e8d6/CDI2013-978202.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/ea58a7598822/CDI2013-978202.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/dcdbb05e1cc9/CDI2013-978202.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6531/3860079/66d508106da9/CDI2013-978202.004.jpg

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