Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, and the Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands.
Extremophiles. 2014 Mar;18(2):331-9. doi: 10.1007/s00792-013-0618-5. Epub 2014 Jan 8.
The majority of Crenarchaeota utilize the cell division system (Cdv) to divide. This system consists of three highly conserved genes, cdvA, cdvB and cdvC that are organized in an operon. CdvC is homologous to the AAA-type ATPase Vps4, involved in multivesicular body biogenesis in eukaryotes. CdvA is a unique archaeal protein that interacts with the membrane, while CdvB is homologous to the eukaryal Vps24 and forms helical filaments. Most Crenarcheota contain additional CdvB paralogs. In Sulfolobus acidocaldarius these are termed CdvB1-3. We have used a gene inactivation approach to determine the impact of these additional cdvB genes on cell division. Independent deletion mutants of these genes were analyzed for growth and protein localization. One of the deletion strains (ΔcdvB3) showed a severe growth defect on plates and delayed growth on liquid medium. It showed the formation of enlarged cells and a defect in DNA segregation. Since these defects are accompanied with an aberrant localization of CdvA and CdvB, we conclude that CdvB3 fulfills an important accessory role in cell division.
大多数古菌利用细胞分裂系统（Cdv）进行分裂。该系统由三个高度保守的基因 cdvA、cdvB 和 cdvC 组成，它们在一个操纵子中组织。CdvC 与真核生物多泡体生物发生中涉及的 AAA 型 ATP 酶 Vps4 同源。CdvA 是一种与膜相互作用的独特古菌蛋白，而 CdvB 与真核 Vps24 同源，并形成螺旋丝。大多数古菌含有额外的 CdvB 旁系同源物。在 Sulfolobus acidocaldarius 中，这些被称为 CdvB1-3。我们使用基因失活方法来确定这些额外的 cdvB 基因对细胞分裂的影响。这些基因的独立缺失突变体被分析用于生长和蛋白质定位。其中一个缺失菌株（ΔcdvB3）在平板上表现出严重的生长缺陷，在液体培养基中生长缓慢。它显示出细胞增大和 DNA 分离缺陷。由于这些缺陷伴随着 CdvA 和 CdvB 的异常定位，我们得出结论，CdvB3 在细胞分裂中起重要的辅助作用。
