Franke W W, Winter S, von Overbeck J, Gudat F, Heitz P U, Stähli C
Virchows Arch A Pathol Anat Histopathol. 1987;411(2):137-47. doi: 10.1007/BF00712737.
The epitope recognized by the murine monoclonal antibody (mAB lu-5) recently described as a formaldehyde-resistant, "pan-epithelial marker" of great value in tumour diagnosis is located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution from amphibia to man. As this epitope is not reactive after SDS-polyacrylamide gel electrophoresis (SDS-PAGE), the epitope-bearing protein has been identified by a dot-blot antibody binding assay, using purified proteins in which the epitope is reconstituted. We show that the epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins. The location of this widespread epitope is discussed with respect to homologies of amino acid sequences of cytokeratins and their conformations.
最近被描述为在肿瘤诊断中具有重要价值的抗甲醛“泛上皮标志物”的鼠单克隆抗体（mAB lu-5）所识别的表位，位于细胞角蛋白丝的表面。从两栖动物到人类的脊椎动物进化过程中，该表位一直得以保留。由于该表位在SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）后无反应性，因此通过斑点印迹抗体结合试验，使用其中表位得以重构的纯化蛋白，鉴定出了携带该表位的蛋白质。我们发现，该表位存在于酸性（I型）和碱性（II型）亚家族的大多数细胞角蛋白多肽中，但在其他细胞骨架蛋白中不存在。结合细胞角蛋白氨基酸序列的同源性及其构象，讨论了这一广泛存在的表位的位置。
