Sadick N S, Swenson P D, Kaufman R L, Kaplan M H
J Am Acad Dermatol. 1987 Jul;17(1):64-9. doi: 10.1016/s0190-9622(87)70172-8.
A study comparing direct immunofluorescence assay using a new monoclonal antibody specific for a varicella-zoster virus glycoprotein complex, the Tzanck smear, and virus isolation for detection of varicella-zoster virus in 56 patients with clinically apparent herpes zoster is presented. Of 47 patients with clinical herpes zoster and with cultures negative for herpes simplex virus, 30 (64%) had positive Tzanck smears, direct immunofluorescence assay results were positive in 26 (55%), and cultures were positive in only 12 (26%). Both direct immunofluorescence assay and the Tzanck smear were found to be superior to culture technics; however, direct immunofluorescence assay was found to have greater specificity.
本文介绍了一项研究，该研究比较了使用一种针对水痘-带状疱疹病毒糖蛋白复合物的新型单克隆抗体的直接免疫荧光测定法、Tzanck涂片法以及病毒分离法，用于检测56例临床诊断为带状疱疹的患者体内的水痘-带状疱疹病毒。在47例临床诊断为带状疱疹且单纯疱疹病毒培养阴性的患者中，30例（64%）Tzanck涂片呈阳性，26例（55%）直接免疫荧光测定结果呈阳性，仅12例（26%）培养结果呈阳性。研究发现，直接免疫荧光测定法和Tzanck涂片法均优于培养技术；然而，直接免疫荧光测定法具有更高的特异性。
