临床实验室尿液分析：UriSed自动显微镜分析仪与手工显微镜检查的比较

Clinical laboratory urine analysis: comparison of the UriSed automated microscopic analyzer and the manual microscopy.

作者信息

Ma Junlong, Wang Chengbin, Yue Jiaxin, Li Mianyang, Zhang Hongrui, Ma Xiaojing, Li Xincui, Xue Dandan, Qing Xiaoyan, Wang Shengjiang, Xiang Daijun, Cong Yulong

机构信息

Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, China.

Wenzhou Medical College, Wenzhou 325035, China.

出版信息

Clin Lab. 2013;59(11-12):1297-303. doi: 10.7754/clin.lab.2013.121128.

DOI:10.7754/clin.lab.2013.121128

PMID:24409664

Abstract

BACKGROUND

Several automated urine sediment analyzers have been introduced to clinical laboratories. Automated microscopic pattern recognition is a new technique for urine particle analysis. We evaluated the analytical and diagnostic performance of the UriSed automated microscopic analyzer and compared with manual microscopy for urine sediment analysis.

METHODS

Precision, linearity, carry-over, and method comparison were carried out. A total of 600 urine samples sent for urinalysis were assessed using the UriSed automated microscopic analyzer and manual microscopy.

RESULTS

Within-run and between-run precision of the UriSed for red blood cells (RBC) and white blood cells (WBC) were acceptable at all levels (CV < 20%). Within-run and between-run imprecision of the UriSed testing for cast, squamous epithelial cells (EPI), and bacteria (BAC) were good at middle level and high level (CV < 20%). The linearity analysis revealed substantial agreement between the measured value and the theoretical value of the UriSed for RBC, WBC, cast, EPI, and BAC (r > 0.95). There was no carry-over. RBC, WBC, and squamous epithelial cells with sensitivities and specificities were more than 80% in this study.

CONCLUSIONS

There is substantial agreement between the UriSed automated microscopic analyzer and the manual microscopy methods. The UriSed provides for a rapid turnaround time.

摘要

背景

几种自动尿沉渣分析仪已被引入临床实验室。自动显微镜模式识别是一种用于尿液颗粒分析的新技术。我们评估了UriSed自动显微镜分析仪的分析和诊断性能，并与手工显微镜检查进行尿沉渣分析比较。

方法

进行了精密度、线性、携带污染和方法比较。共使用UriSed自动显微镜分析仪和手工显微镜检查对600份送检尿液样本进行了评估。

结果

UriSed对红细胞（RBC）和白细胞（WBC）的批内和批间精密度在所有水平均可接受（CV<20%）。UriSed对管型、鳞状上皮细胞（EPI）和细菌（BAC）检测的批内和批间不精密度在中高水平良好（CV<20%）。线性分析显示UriSed对RBC、WBC、管型、EPI和BAC的测量值与理论值之间有高度一致性（r>0.95）。无携带污染。本研究中，RBC、WBC和鳞状上皮细胞的敏感性和特异性均超过80%。