Aydin Ozgur, Ellidag Hamit Yasar, Eren Esin, Yilmaz Necat
Clinical Biochemistry, Batman Maternity and Children's Hospital, Batman, Turkey.
Central Laboratories, Antalya Education and Research Hospital, Antalya, Turkey.
J Med Biochem. 2015 Jul;34(3):332-337. doi: 10.2478/jomb-2014-0052. Epub 2015 Jul 14.
Automated urine sediment analyzers have proven their feasibility in medical laboratories. However, editing manual microscopic review of some specimens severely limits the usefulness of such systems. This study aims to give feedback on the practical experience on »Yeast«, which is one of the parameters that compel frequent manual reviews.
5448 freshly collected urine specimens submitted from various departments of our hospital for diagnostic urinalysis were studied by the UriSed® (77 Elektronika, Hungary). A specialist medical doctor inspected every image on-board, and reviewed the ones with a »Yeast« alarm by traditional manual microscopy.
UriSed alarmed in 491 samples (9%) for yeast. In 59 samples (1%) the number of particles exceeded the cut-off and a »positive for yeast« was set. A false positive report of yeast +1 to 3+/HPF was found in 51 samples (0.9%). There were 8 cases with positive for yeast from both microscopic methods. Thirty-three »negative for yeast« samples were corrected as positive after the manual microscopic review.
We report a high percentage of false positives and negatives in the yeast parameter, in line with other studies on UriSed as well as on other instruments in the market. As an important feedback, our observations showed that the major concern in false results was »the focusing problem«. We believe in the necessity of a focus check and comparison of alarms between images on board.
自动尿沉渣分析仪已在医学实验室中证明了其可行性。然而，对某些标本进行手工显微镜复查严重限制了此类系统的实用性。本研究旨在就“酵母”这一参数的实际经验提供反馈，该参数常需频繁进行手工复查。
我们使用UriSed®（匈牙利77 Elektronika公司）对我院各科室提交的5448份新鲜采集的尿液标本进行诊断性尿液分析研究。一名专科医生检查了仪器上的每一张图像，并通过传统手工显微镜对发出“酵母”警报的图像进行复查。
UriSed对491份样本（9%）发出酵母警报。59份样本（1%）中的颗粒数量超过临界值，并判定为“酵母阳性”。在51份样本（0.9%）中发现酵母假阳性报告为+1至3+/HPF。两种显微镜检查方法均有8例酵母阳性病例。33份“酵母阴性”样本经手工显微镜复查后更正为阳性。
我们报告酵母参数的假阳性和假阴性比例较高，这与其他关于UriSed以及市场上其他仪器的研究结果一致。作为一项重要反馈，我们的观察结果表明，错误结果的主要问题是“聚焦问题”。我们认为有必要进行聚焦检查并比较仪器上各图像之间的警报。
