Key Laboratory of Eutrophication and Red Tide Prevention, Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou, 510632, China.
Chinese Sturgeon Research Institute, Three Gorges Corporation, Yichang, 443100, China.
Sci Rep. 2017 May 8;7(1):1553. doi: 10.1038/s41598-017-01654-y.
The mitochondrial genome (mitogenome) can provide useful information for analyzing phylogeny and molecular evolution. Scorpaeniformes is one of the most diverse teleostean orders and has great commercial importance. To develop mitogenome data for this important group, we determined the complete mitogenomes of two gurnards Chelidonichthys kumu and Lepidotrigla microptera of Triglidae within Scorpaeniformes for the first time. The mitogenomes are 16,495 bp long in C. kumu and 16,610 bp long in L. microptera. Both the mitogenomes contain 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and two non-coding regions. All PCGs are initiated by ATG codons, except for the cytochrome coxidase subunit 1 (cox1) gene. All of the tRNA genes could be folded into typical cloverleaf secondary structures, with the exception of tRNA lacks a dihydrouracil (DHU) stem. The control regions are both 838 bp and contain several features common to Scorpaeniformes. The phylogenetic relationships of 33 fish mitogenomes using Bayesian Inference (BI) and Maximum Likelihood (ML) based on nucleotide and amino acid sequences of 13 PCGs indicated that the mitogenome sequences could be useful in resolving higher-level relationship of Scorpaeniformes. The results may provide more insight into the mitogenome evolution of teleostean species.
线粒体基因组（mitogenome）可以为分析系统发育和分子进化提供有用的信息。鲉形目是最具多样性的硬骨鱼目之一，具有重要的商业价值。为了为这个重要的群体开发线粒体基因组数据，我们首次确定了鲉形目鲂鮄科的两种鲂鮄（Chelidonichthys kumu）和细鳞鲂（Lepidotrigla microptera）的完整线粒体基因组。C. kumu 的线粒体基因组长 16,495bp，L. microptera 的线粒体基因组长 16,610bp。这两个线粒体基因组都包含 13 个蛋白质编码基因（PCGs）、2 个核糖体 RNA（rRNA）基因、22 个转移 RNA（tRNA）基因和两个非编码区。除细胞色素 coxidase 亚基 1（cox1）基因外，所有 PCGs 均以 ATG 密码子起始。所有 tRNA 基因都可以折叠成典型的三叶形二级结构，除了 tRNA 缺乏二氢尿嘧啶（DHU）茎。控制区均为 838bp，包含鲉形目共有的几个特征。基于 13 个 PCGs 的核苷酸和氨基酸序列，使用贝叶斯推断（BI）和最大似然法（ML）对 33 种鱼类线粒体基因组的系统发育关系进行分析表明，线粒体基因组序列可用于解决鲉形目更高层次的关系。研究结果可能为硬骨鱼类的线粒体基因组进化提供更多的见解。
