University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain.
Parasitol Res. 2014 Apr;113(4):1383-8. doi: 10.1007/s00436-014-3778-z. Epub 2014 Jan 22.
Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains in soil samples collected between 2012 and 2013 in Gran Canaria Island, Canary Islands, Spain. Samples were inoculated onto non-nutrient agar (NNA) plates and were checked for the presence of Acanthamoeba. Identification of Acanthamoeba strains was based on the morphology of the cyst and trophozoite forms. Subsequently, positive samples were cloned for their molecular characterization at the genotype level by sequencing the DF3 region located in the 18S rDNA gene of Acanthamoeba as previously described. Sequencing results revealed the presence of T2, T5 and T4 genotypes within the studied samples. To the best of our knowledge, this is the first report demonstrating the presence of Acanthamoeba in Gran Canaria Island and the first study at the genotype level in the Canary Islands.
自由生活的棘阿米巴属包括非致病性和致病性菌株，目前分为 18 个不同的基因型，T1-T18。本研究对西班牙加那利群岛大加那利岛 2012 年至 2013 年间采集的土壤样本进行了调查，以评估棘阿米巴菌株的存在情况。将样本接种到非营养琼脂（NNA）平板上，检查是否存在棘阿米巴。根据囊和滋养体形态鉴定棘阿米巴菌株。随后，通过对先前描述的位于棘阿米巴 18S rDNA 基因的 DF3 区域进行测序，对阳性样本进行克隆，以在基因型水平上进行分子特征分析。测序结果显示，在所研究的样本中存在 T2、T5 和 T4 基因型。据我们所知，这是首次报道在大加那利岛发现棘阿米巴，也是在加那利群岛进行的首次基因型水平研究。
