Gere Jephris, Yessoufou Kowiyou, Daru Barnabas H, Mankga Ledile T, Maurin Olivier, van der Bank Michelle
African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, PO Box 524, South Africa.
African Centre for DNA Barcoding, Department of Botany & Plant Biotechnology, University of Johannesburg, PO Box 524, South Africa ; C4 EcoSolutions, 9 Mohr Road Tokai, Cape Town, South Africa 7945.
Zookeys. 2013 Dec 30(365):129-47. doi: 10.3897/zookeys.365.5728.
Recent studies indicate that the discriminatory power of the core DNA barcodes (rbcLa + matK) for land plants may have been overestimated since their performance have been tested only on few closely related species. In this study we focused mainly on how the addition of complementary barcodes (nrITS and trnH-psbA) to the core barcodes will affect the performance of the core barcodes in discriminating closely related species from family to section levels. In general, we found that the core barcodes performed poorly compared to the various combinations tested. Using multiple criteria, we finally advocated for the use of the core + trnH-psbA as potential DNA barcode for the family Combretaceae at least in southern Africa. Our results also indicate that the success of DNA barcoding in discriminating closely related species may be related to evolutionary and possibly the biogeographic histories of the taxonomic group tested.
最近的研究表明，核心DNA条形码（rbcLa + matK）对陆地植物的鉴别能力可能被高估了，因为其性能仅在少数亲缘关系较近的物种上进行过测试。在本研究中，我们主要关注在核心条形码中添加互补条形码（nrITS和trnH-psbA）如何影响核心条形码在区分从科到组水平的近缘物种时的性能。总体而言，我们发现与测试的各种组合相比，核心条形码表现不佳。使用多种标准，我们最终主张至少在南部非洲将核心 + trnH-psbA用作使君子科潜在的DNA条形码。我们的结果还表明，DNA条形码在区分近缘物种方面的成功可能与所测试分类群的进化以及可能的生物地理历史有关。
