Fultz P N, Srinivasan A, Greene C R, Butler D, Swenson R B, McClure H M
AIDS Program, Centers for Disease Control, Atlanta, Georgia 30333.
J Virol. 1987 Dec;61(12):4026-9. doi: 10.1128/JVI.61.12.4026-4029.1987.
Two chimpanzees, one (C-499) infected with the acquired immunodeficiency syndrome-associated retrovirus type 2 (ARV-2) strain of human immunodeficiency virus (HIV) and one (C-560) infected with the lymphadenopathy-associated virus type 1 (LAV-1) strain of HIV, were inoculated with approximately 10(4) tissue culture infective doses of the reciprocal strain. At the time of the second inoculation, both chimpanzees had high titers of HIV-specific antibodies, including antibodies that neutralized both virus strains. After inoculation of the second strain of HIV, the antibody titers in both chimpanzees increased 4- to 10-fold, and in one chimpanzee (C-499), the numbers of infectious peripheral blood mononuclear cells (PBMC) increased 1,000-fold to levels that are comparable with those observed after primary HIV infections. By restriction enzyme analysis of virus recovered from PBMC, both ARV-2 and LAV-1 were identified in C-499, thus demonstrating that superinfection had occurred.
两只黑猩猩，一只（C - 499）感染了人类免疫缺陷病毒（HIV）的2型获得性免疫缺陷综合征相关逆转录病毒（ARV - 2）毒株，另一只（C - 560）感染了HIV的1型淋巴结病相关病毒（LAV - 1）毒株，分别接种了约10⁴组织培养感染剂量的相应毒株。在第二次接种时，两只黑猩猩都有高滴度的HIV特异性抗体，包括能中和两种病毒毒株的抗体。接种第二种HIV毒株后，两只黑猩猩的抗体滴度均增加了4至10倍，并且在一只黑猩猩（C - 499）中，感染性外周血单个核细胞（PBMC）数量增加了1000倍，达到了与初次HIV感染后观察到的水平相当的程度。通过对从PBMC中回收的病毒进行限制性酶切分析，在C - 499中鉴定出了ARV - 2和LAV - 1，从而证明发生了双重感染。
