Druart Céline, Dewulf Evelyne M, Cani Patrice D, Neyrinck Audrey M, Thissen Jean-Paul, Delzenne Nathalie M
Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Avenue E. Mounier 73, PO box B1.73.11, 1200, Brussels, Belgium.
Lipids. 2014 Apr;49(4):397-402. doi: 10.1007/s11745-014-3881-z. Epub 2014 Jan 29.
The aim of this human study was to assess the influence of prebiotic-induced gut microbiota modulation on PUFA-derived bacterial metabolites production. Therefore, we analyzed the circulating fatty acid profile including CLA/CLnA in obese women treated during 3 months with inulin-type fructan prebiotics. In these patients, we had already determined gut microbiota composition by phylogenetic microarray and qPCR analysis of 16S rDNA. Some PUFA-derived bacterial metabolites were detected in the serum of obese patients. Despite the prebiotic-induced modulation of gut microbiota, including changes in CLA/CLnA-producing bacteria, the treatment did not impact significantly on the circulating level of these metabolites. However, some PUFA-derived bacterial metabolites were positively correlated with specific fecal bacteria (Bifidobacterium spp., Eubacterium ventriosum and Lactobacillus spp.) and inversely correlated with serum cholesterol (total, LDL, HDL). These correlations suggest a potential beneficial effect of some of these metabolites but this remains to be confirmed by further investigation.
这项人体研究的目的是评估益生元诱导的肠道微生物群调节对多不饱和脂肪酸（PUFA）衍生的细菌代谢产物生成的影响。因此，我们分析了肥胖女性在接受菊粉型果聚糖益生元治疗3个月期间的循环脂肪酸谱，包括共轭亚油酸（CLA）/共轭亚麻酸（CLnA）。在这些患者中，我们已经通过系统发育微阵列和16S核糖体DNA的定量聚合酶链反应（qPCR）分析确定了肠道微生物群的组成。在肥胖患者的血清中检测到了一些PUFA衍生的细菌代谢产物。尽管益生元诱导了肠道微生物群的调节，包括CLA/CLnA产生菌的变化，但该治疗对这些代谢产物的循环水平没有显著影响。然而，一些PUFA衍生的细菌代谢产物与特定的粪便细菌（双歧杆菌属、膨胀真杆菌和乳酸杆菌属）呈正相关，与血清胆固醇（总胆固醇、低密度脂蛋白、高密度脂蛋白）呈负相关。这些相关性表明其中一些代谢产物可能具有有益作用，但这仍有待进一步研究证实。
