Yasui Takahiro, Kobayashi Takahiro, Okada Atsushi, Hamamoto Shuzo, Hirose Masahito, Mizuno Kentaro, Kubota Yasue, Umemoto Yukihiro, Kawai Noriyasu, Tozawa Keiichi, Gao Bing, Kohri Kenjiro
Department of Nephro-urology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
BMC Res Notes. 2014 Feb 8;7:87. doi: 10.1186/1756-0500-7-87.
Melamine was recently identified as a risk factor for renal calculi following the milk powder contamination in China. However, the long-term natural history of melamine exposure and its renal effects remain unknown. We evaluated renal function and other adverse health effects using a rat model administered melamine and cyanuric aid, considering age and sex.
Twelve male F334/N rats each of ages 6, 10, and 26 weeks (N = 36) were equally assigned to Group M + C or controls. Group M + C rats were administered 12 mg · kg(-1) · day(-1) of melamine and cyanuric acid for 28 days. Serum and urine samples and kidney sections were evaluated on day 28. Six-week-old male and female F344/N rats were administered 12 mg of melamine and cyanuric acid for 28 days. Body weights were measured weekly; on days 0, 28, 90, and 180 after the 28-day period of melamine and cyanuric acid administration, serum samples and kidney sections were obtained.
Although the control group had no crystals, 6-week-old Group M + C rats had more crystals compared to the 10- and 26-week old Group M + C rats. Male rats also had significantly more crystals than females of the same age. Male rats were affected to a greater extent than females.
Younger rats experienced more severe renal failure and greater renal crystal deposition following melamine and cyanuric acid administration. However, after melamine and cyanuric acid administration cessation, crystal deposition and renal failure improved and did not cause growth arrest. Therefore, early diagnosis of melamine-associated calculi is critical.
在中国奶粉被三聚氰胺污染事件后，三聚氰胺最近被确定为肾结石的一个风险因素。然而，三聚氰胺暴露的长期自然史及其对肾脏的影响仍不清楚。我们使用三聚氰胺和氰尿酸给药的大鼠模型，考虑年龄和性别，评估肾功能及其他不良健康影响。
将12只6周龄、10周龄和26周龄的雄性F344/N大鼠（N = 36）平均分为三聚氰胺+氰尿酸组（M + C组）或对照组。M + C组大鼠每天给予12 mg·kg⁻¹的三聚氰胺和氰尿酸，持续28天。在第28天评估血清、尿液样本和肾脏切片。对6周龄的雄性和雌性F344/N大鼠给予12 mg的三聚氰胺和氰尿酸，持续28天。每周测量体重；在给予三聚氰胺和氰尿酸28天的时间段后的第0、28、90和180天，采集血清样本和肾脏切片。
虽然对照组没有晶体，但与10周龄和26周龄的M + C组大鼠相比，6周龄的M + C组大鼠有更多晶体。雄性大鼠的晶体也明显多于同年龄的雌性大鼠。雄性大鼠比雌性大鼠受影响的程度更大。
较年轻的大鼠在给予三聚氰胺和氰尿酸后经历了更严重的肾衰竭和更多的肾脏晶体沉积。然而，在停止给予三聚氰胺和氰尿酸后，晶体沉积和肾衰竭有所改善，且未导致生长停滞。因此，三聚氰胺相关结石的早期诊断至关重要。
