Menshawy Ahmed M S, Perez-Sancho Marta, Garcia-Seco Teresa, Hosein Hosein I, García Nerea, Martinez Irene, Sayour Ashraf E, Goyache Joaquín, Azzam Ragab A A, Dominguez Lucas, Alvarez Julio
Faculty of Veterinary Medicine, Beni-Suef University, Shamlaa Street, Beni-Suef 62511, Egypt.
Centro VISAVET, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, Spain ; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, Spain.
Biomed Res Int. 2014;2014:353876. doi: 10.1155/2014/353876. Epub 2014 Jan 6.
Brucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 Brucella spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002-2007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as B. melitensis biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as B. abortus (n = 2) and B. suis biovar 1 (n = 2). MLVA-15 yielded a high discriminatory power (h = 0.801), indicating a high genetic diversity among the B. melitensis strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of B. suis from cattle in Egypt which, coupled with the finding of B. abortus, suggests a potential role of livestock as reservoirs of several zoonotic Brucella species in the region.
布鲁氏菌病在埃及大部分地区呈地方性流行，主要由马尔他布鲁氏菌生物变种3引起，尽管一直在努力控制，但仍影响着牛和小反刍动物。了解某一地区主要流行的布鲁氏菌种类/菌株是制定布鲁氏菌病控制策略的前提条件。因此，开展了一项研究，旨在通过微生物学检测和分子生物学技术（聚合酶链反应、15个可变数目串联重复序列多位点可变数目串联重复序列分析和测序），评估在五年期间（2002 - 2007年）从四个省份的家养反刍动物（牛、水牛、绵羊和山羊）中分离出的17株布鲁氏菌属菌株的表型和遗传异质性。13株菌株被鉴定为马尔他布鲁氏菌生物变种3，而所有表型和遗传技术均将其余分离株分类为流产布鲁氏菌（n = 2）和猪布鲁氏菌生物变种1（n = 2）。15个可变数目串联重复序列多位点可变数目串联重复序列分析具有较高的鉴别力（h = 0.801），表明在埃及家养反刍动物中流行的马尔他布鲁氏菌菌株间存在高度遗传多样性。这是埃及首次从牛中分离出猪布鲁氏菌的报告，再加上流产布鲁氏菌的发现，表明家畜在该地区可能是几种人畜共患布鲁氏菌的宿主。
