Turley H, Pulford K A, Gatter K C, Mason D Y
Nuffield Department of Pathology, John Radcliffe Hospital, Oxford, UK.
Br J Exp Pathol. 1988 Jun;69(3):433-40.
Recent immunocytochemical studies have revealed that cytokeratin intermediate filaments, previously thought to be restricted to epithelial tissues, are present in muscle. In view of the implications of these reports for diagnostic pathology it is important to investigate by biochemical means whether these findings represent the presence of true cytokeratin proteins or an unexpected antigenic cross-reaction. In the present study intermediate filament proteins have been extracted from samples of human myometrium and identified by immunoblotting techniques using well characterized monoclonal antibodies, two against cytokeratins and two against desmin. The results show that the proteins of the appropriate molecular weight for cytokeratin were labelled by anti-cytokeratin antibodies and were quite distinct from those recognized by anti-desmin antibodies. This study therefore confirms previous immunocytochemical findings and emphasizes the need for caution when using anti-intermediate filament antibodies for diagnostic purposes.
最近的免疫细胞化学研究表明，细胞角蛋白中间丝以前被认为仅限于上皮组织，现在发现其也存在于肌肉中。鉴于这些报告对诊断病理学的影响，通过生化方法研究这些发现是代表真正的细胞角蛋白蛋白的存在还是意外的抗原交叉反应很重要。在本研究中，中间丝蛋白已从人子宫肌层样本中提取，并使用特征明确的单克隆抗体通过免疫印迹技术进行鉴定，其中两种针对细胞角蛋白，两种针对结蛋白。结果表明，抗细胞角蛋白抗体标记了具有适合细胞角蛋白分子量的蛋白质，这些蛋白质与抗结蛋白抗体识别的蛋白质截然不同。因此，本研究证实了先前的免疫细胞化学发现，并强调在将抗中间丝抗体用于诊断目的时需要谨慎。
