Mukherji Ruchira, Prabhune Asmita
Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India.
ScientificWorldJournal. 2014 Jan 14;2014:890709. doi: 10.1155/2014/890709. eCollection 2014.
Essential oils (EOs) form an important part of traditional medicine so their anti-microbial and, in the recent past, antiquorum sensing activity has been well studied. However it is likely that due to their hydrophobic nature and reduced solubility in aqueous environments full potential of their activity cannot be realized. hence it is only rational to formulate a process to make these molecules more polar in nature. The present paper reports synthesis of sophorolipids using 12 different essential oils as substrates, thus providing surfactant-like properties to these EOs. The synthesis protocol makes the use of Candida bombicola ATCC 22214 as producer organism. The production process required 7 days of incubation at 28°C and 180 rpm. Preliminary characterization of the synthesized essential oil sophorolipids (EOSLs) was performed using thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). Additionally, essential oils that were incapable of mediating quorum sensing inhibition (QSI) on their own became potent quorum sensing inhibitors upon conversion into their corresponding EOSLs. Antibiofilm potential of these EOSLs was also demonstrated using V. cholerae as test organism. Use of essential oils as substrates for glycolipid synthesis has not been attempted previously, and hence this is the first report.
精油（EOs）是传统医学的重要组成部分，因此其抗菌活性以及最近发现的群体感应抑制活性已得到充分研究。然而，由于其疏水性以及在水性环境中溶解度降低，其活性的全部潜力可能无法实现。因此，制定一个使这些分子在性质上更具极性的过程是合理的。本文报道了以12种不同的精油为底物合成槐糖脂，从而赋予这些精油类似表面活性剂的性质。合成方案使用解脂耶氏酵母ATCC 22214作为生产菌株。生产过程需要在28°C和180 rpm下培养7天。使用薄层色谱（TLC）和傅里叶变换红外光谱（FTIR）对合成的精油槐糖脂（EOSLs）进行了初步表征。此外，那些自身无法介导群体感应抑制（QSI）的精油在转化为相应的EOSLs后成为有效的群体感应抑制剂。还以霍乱弧菌为测试菌株证明了这些EOSLs的抗生物膜潜力。以前尚未尝试使用精油作为糖脂合成的底物，因此这是首次报道。
