基于聚腺苷酸聚合酶的聚腺苷酸长度测定
Poly(A) polymerase-based poly(A) length assay.
作者信息
Patil Deepak P, Bakthavachalu Baskar, Schoenberg Daniel R
机构信息
Center for RNA Biology, The Ohio State University, Columbus, OH, USA.
出版信息
Methods Mol Biol. 2014;1125:13-23. doi: 10.1007/978-1-62703-971-0_2.
Abstract
mRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.
摘要
信使核糖核酸（mRNA）的多聚腺苷酸化作用于核输出、翻译及稳定性过程。我们描述了一种高效的实验方案，该方案旨在评估聚腺苷酸（poly(A)）尾的长度，其基于酵母多聚(A)聚合酶进行3'加尾，并通过毛细管电泳对产物进行单核苷酸分辨率的分析。
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