Pacurar Daniel Ioan, Pacurar Monica Lacramioara, Bussell John Desmond, Schwambach Joseli, Pop Tiberia Ioana, Kowalczyk Mariusz, Gutierrez Laurent, Cavel Emilie, Chaabouni Salma, Ljung Karin, Fett-Neto Arthur Germano, Pamfil Doru, Bellini Catherine
Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187 Umeå, Sweden.
J Exp Bot. 2014 Apr;65(6):1605-18. doi: 10.1093/jxb/eru026. Epub 2014 Mar 4.
The plant hormone auxin plays a central role in adventitious rooting and is routinely used with many economically important, vegetatively propagated plant species to promote adventitious root initiation and development on cuttings. Nevertheless the molecular mechanisms through which it acts are only starting to emerge. The Arabidopsis superroot2-1 (sur2-1) mutant overproduces auxin and, as a consequence, develops excessive adventitious roots in the hypocotyl. In order to increase the knowledge of adventitious rooting and of auxin signalling pathways and crosstalk, this study performed a screen for suppressors of superroot2-1 phenotype. These suppressors provide a new resource for discovery of genetic players involved in auxin signalling pathways or at the crosstalk of auxin and other hormones or environmental signals. This study reports the identification and characterization of 26 sur2-1 suppressor mutants, several of which were identified as mutations in candidate genes involved in either auxin biosynthesis or signalling. In addition to confirming the role of auxin as a central regulator of adventitious rooting, superroot2 suppressors indicated possible crosstalk with ethylene signalling in this process.
植物激素生长素在不定根形成过程中起核心作用，并且经常被用于许多具有重要经济价值的无性繁殖植物物种，以促进插条上不定根的起始和发育。然而，其作用的分子机制才刚刚开始显现。拟南芥超根2-1（sur2-1）突变体过量产生生长素，结果在胚轴上形成过多的不定根。为了增加对不定根形成以及生长素信号通路和相互作用的了解，本研究对超根2-1表型的抑制子进行了筛选。这些抑制子为发现参与生长素信号通路或生长素与其他激素或环境信号相互作用的基因参与者提供了新的资源。本研究报告了26个sur2-1抑制子突变体的鉴定和表征，其中几个被鉴定为参与生长素生物合成或信号传导的候选基因中的突变。除了证实生长素作为不定根形成的核心调节因子的作用外，超根2抑制子还表明在此过程中可能与乙烯信号发生相互作用。
