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伪装解释的细胞学假象。

Cytological artifacts masquerading interpretation.

作者信息

Sahay Khushboo, Mehendiratta Monica, Rehani Shweta, Kumra Madhumani, Sharma Rashi, Kardam Priyanka

机构信息

Department of Oral Pathology and Microbiology, Sudha Rustagi College of Dental Sciences and Hospital, Faridabad, Haryana, India.

出版信息

J Cytol. 2013 Oct;30(4):241-6. doi: 10.4103/0970-9371.126649.

DOI:10.4103/0970-9371.126649
PMID:24648667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3945624/
Abstract

BACKGROUND

Cytological artifacts are important to learn because an error in routine laboratory practice can bring out an erroneous result.

AIMS

The aim of this study was to analyze the effects of delayed fixation and morphological discrepancies created by deliberate addition of extraneous factors on the interpretation and/or diagnosis of an oral cytosmear.

MATERIALS AND METHODS

A prospective study was carried out using papanicolaou and hematoxylin and eosin-stained oral smears, 6 each from 66 volunteer dental students with deliberate variation in fixation delay timings, with and without changes in temperature, undue pressure while smear making and intentional addition of contaminants. The fixation delay at room temperature was carried out at an interval of every 30 minutes, 1 day and 1 week and was continued till the end of 1 day, 1 week, and 1 month, respectively. The temperature variations included 60 to 70°C and 3 to 4°C.

RESULTS

Light microscopically, the effect of delayed fixation at room temperature appeared first on cytoplasm followed by nucleus within the first 2 hours and on the 4(th) day, respectively, till complete cytoplasmic degeneration on the 23(rd) day. However, delayed fixation at variable temperature brought faster degenerative changes at higher temperature than lower temperature. Effect of extraneous factors revealed some interesting facts.

CONCLUSIONS

In order to justify a cytosmear interpretation, a cytologist must be well acquainted with delayed fixation-induced cellular changes and microscopic appearances of common contaminants so as to implicate better prognosis and therapy.

摘要

背景

了解细胞学假象很重要，因为常规实验室操作中的一个错误可能会导致错误结果。

目的

本研究的目的是分析延迟固定以及故意添加外部因素所造成的形态差异对口腔细胞涂片解读和/或诊断的影响。

材料与方法

采用巴氏染色和苏木精-伊红染色的口腔涂片进行前瞻性研究，从66名志愿牙科学生中每人获取6张涂片，故意改变固定延迟时间，包括有无温度变化、涂片制作时施加不当压力以及故意添加污染物。在室温下每隔30分钟、1天和1周进行一次固定延迟，并分别持续到1天、1周和1个月结束。温度变化包括60至70°C和3至4°C。

结果

在光学显微镜下，室温下延迟固定的影响首先在2小时内出现在细胞质上，然后在第4天出现在细胞核上，直到第23天细胞质完全退化。然而，在不同温度下延迟固定，较高温度比较低温度会带来更快的退化变化。外部因素的影响揭示了一些有趣的事实。

结论

为了合理地解读细胞涂片，细胞学家必须充分了解延迟固定引起的细胞变化以及常见污染物的微观表现，以便做出更好的预后和治疗判断。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2ff/3945624/916df68379c1/JCytol-30-241-g018.jpg
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本文引用的文献

1
The consequence of delayed fixation on subsequent preservation of urine cells.延迟固定对尿液细胞后续保存的影响。
Oman Med J. 2011 Jan;26(1):14-8. doi: 10.5001/omj.2011.04.
2
Maltese cross: Starch artifact in oral cytology, divulged through polarized microscopy.马尔他十字：口腔细胞学中的淀粉样伪像，通过偏振显微镜揭示。
J Cytol. 2010 Jan;27(1):40-1. doi: 10.4103/0970-9371.66698.
3
Application of cytology and molecular biology in diagnosing premalignant or malignant oral lesions.细胞学与分子生物学在口腔癌前病变或恶性病变诊断中的应用。
J Pathol Inform. 2017 Apr 10;8:19. doi: 10.4103/jpi.jpi_94_16. eCollection 2017.
Mol Cancer. 2006 Mar 23;5:11. doi: 10.1186/1476-4598-5-11.
4
Facts about artefacts in diagnostic pathology.诊断病理学中人工制品的相关事实。
Indian J Pathol Microbiol. 2002 Jan;45(1):133-5.
5
Prevalence, identification and significance of fiber contaminants in cervical smears.宫颈涂片纤维污染物的患病率、识别及意义
Acta Cytol. 1996 May-Jun;40(3):489-95. doi: 10.1159/000333904.