Okmen G, Turkcan O
Mugla Sıtkı Kocman University, Faculty of Science, Department of Biology, TURKEY.
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):116-20. doi: 10.4314/ajtcam.v11i1.17. eCollection 2014.
[corrected] The aim of this work was to investigate the antimicrobial, antioxidant, and antimutagenic potentials of methanol extracts from E. angustifolia.
Methanol extracts were screened for antimicrobial activity against different species of 4 Gram positive and 3 Gram negative bacteria and one fungus. These bacteria included food pathogens. The leaf extract was tested using disc diffusion assay.
The methanol extract of E. angustifolia showed maximum inhibition zone of 16 mm against Yersinia enterocolitica. Whereas, the inhibition zone was not determined by methanol extract against Escherichia coli ATCC 1122 and Candida albicans RSKK 02029. The MIC was evaluated on plant extracts as antimicrobial activity. All of bacterial strains showed the lowest sensitivity to methanol extract of E. angustifolia (3.5 mg/mL), except Yersinia enterocolitica NCTC 11174. In addition, the plant extracts were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical. Finally, the methanol extract displayed a strong antioxidant activity (Trolox equivalent = 1.49 mM). Also, E. angustifolia methanol extracts were screened for their antimutagenic activity against sodium azide by Ames test in absence of rat microsomal liver enzyme (-S9). The results showed that E. angustifolia methanol extracts can inhibit mutagenic agents of sodium azide. The plant leaf extracts with the inhibition of 36% sodium azide showed moderate potential in decreasing mutagenic agents in Salmonella typhimurium TA100.
E. angustifolia methanol extracts have antimicrobial, antioxidant and antimutagenic potential.
[已修正] 本研究旨在探究狭叶紫锥菊甲醇提取物的抗菌、抗氧化和抗诱变潜力。
对甲醇提取物针对4种革兰氏阳性菌、3种革兰氏阴性菌和1种真菌的不同菌株进行抗菌活性筛选。这些细菌包括食源性病原体。采用纸片扩散法对叶提取物进行检测。
狭叶紫锥菊甲醇提取物对小肠结肠炎耶尔森菌的最大抑菌圈为16毫米。然而，甲醇提取物对大肠埃希菌ATCC 1122和白色念珠菌RSKK 02029未检测到抑菌圈。以最低抑菌浓度评估植物提取物的抗菌活性。除小肠结肠炎耶尔森菌NCTC 11174外，所有菌株对狭叶紫锥菊甲醇提取物的敏感性最低（3.5毫克/毫升）。此外，对植物提取物进行稳定的2,2-二苯基-1-苦基肼自由基（DPPH）清除能力测试。最终，甲醇提取物表现出较强的抗氧化活性（Trolox当量 = 1.49毫摩尔）。同时，通过在无大鼠微粒体肝酶（-S9）条件下的艾姆斯试验，对狭叶紫锥菊甲醇提取物针对叠氮化钠的抗诱变活性进行筛选。结果表明，狭叶紫锥菊甲醇提取物可抑制叠氮化钠诱变剂。在鼠伤寒沙门氏菌TA100中，对叠氮化钠抑制率为36%的植物叶提取物在降低诱变剂方面显示出中等潜力。
狭叶紫锥菊甲醇提取物具有抗菌、抗氧化和抗诱变潜力。
