Xuan Ning, Guo Xia, Xie Hong-Yan, Lou Qi-Nian, Lu Xing-Bo, Liu Guo-Xia, Picimbon Jean-François
Biotechnology Research Center, Shandong Provincial Key Laboratory for Genetic Improvement Cultivation, Ecology and Physiology of Crops, Shandong Academy of Agricultural Sciences, Jinan.
Insect Sci. 2015 Apr;22(2):203-19. doi: 10.1111/1744-7917.12116. Epub 2014 Apr 17.
We analyzed 20 chemosensory protein (CSP) genes of the silkworm Bombyx mori. We found a high number of retrotransposons inserted in introns. We then analyzed expression of the 20 BmorCSP genes across tissues using quantitative real-time polymerase chain reaction (PCR). Relatively low expression levels of BmorCSPs were found in the gut and fat body tissues. We thus tested the effects of endectocyte insecticide abamectin (B1a and B1b avermectins) on BmorCSP gene expression. Quantitative real-time PCR experiments showed that a single brief exposure to insecticide abamectin increased dramatically CSP expression not only in the antennae but in most tissues, including gut and fat body. Furthermore, our study showed coordinate expression of CSPs and metabolic cytochrome P450 enzymes in a tissue-dependent manner in response to the insecticide. The function of CSPs remains unknown. Based on our results, we suggest a role in detecting xenobiotics that are then detoxified by cytochrome P450 anti-xenobiotic enzymes.
我们分析了家蚕（Bombyx mori）的20个化学感受蛋白（CSP）基因。我们发现大量反转录转座子插入到内含子中。然后，我们使用定量实时聚合酶链反应（PCR）分析了这20个家蚕CSP基因在不同组织中的表达情况。结果发现，家蚕CSP基因在肠道和脂肪体组织中的表达水平相对较低。因此，我们测试了内吸性杀虫剂阿维菌素（B1a和B1b阿维菌素）对家蚕CSP基因表达的影响。定量实时PCR实验表明，单次短暂接触杀虫剂阿维菌素不仅会使触角中的CSP表达显著增加，在包括肠道和脂肪体在内的大多数组织中也是如此。此外，我们的研究表明，CSP与代谢性细胞色素P450酶在组织依赖的方式下协同表达以响应杀虫剂。CSP的功能仍然未知。基于我们的研究结果，我们认为CSP在检测外源性物质方面发挥作用，这些外源性物质随后会被细胞色素P450抗外源性物质酶解毒。
