用于检测1型人类免疫缺陷病毒复制的核心抗原（p24）检测法与逆转录酶活性的比较。
Comparison of core antigen (p24) assay and reverse transcriptase activity for detection of human immunodeficiency virus type 1 replication.
作者信息
Land S, Beaton F, McPhee D A, Gust I D
机构信息
Virology Department, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital for Communicable Diseases, Victoria, Australia.
出版信息
J Clin Microbiol. 1989 Mar;27(3):486-9. doi: 10.1128/jcm.27.3.486-489.1989.
Abstract
This report compares two assay systems for monitoring human immunodeficiency virus (HIV) replication in peripheral blood leukocyte cultures. A commercial enzyme-linked immunoassay detected core antigen (p24) in 80% of cell cultures from HIV-seropositive individuals, whereas 67% of the cell cultures produced detectable levels of reverse transcriptase activity. There were clearly three patterns of reverse transcriptase activity produced, two of which may evade detection without regular sampling and maintaining cell cultures for more than 4 weeks. Once established, core antigen levels remained high so that cell cultures could be confidently monitored by an intermittent screening regimen.
摘要
本报告比较了两种用于监测外周血白细胞培养物中人类免疫缺陷病毒（HIV）复制的检测系统。一种商业酶联免疫测定法在80%的HIV血清阳性个体的细胞培养物中检测到核心抗原（p24），而67%的细胞培养物产生了可检测水平的逆转录酶活性。所产生的逆转录酶活性显然有三种模式，其中两种模式如果不定期取样并维持细胞培养超过4周，可能会逃避检测。一旦确立，核心抗原水平会保持在较高水平，因此可以通过间歇性筛查方案可靠地监测细胞培养物。
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