Lee Jong Seo, Baek Sang Dae, Venkatesan Jayachandran, Bhatnagar Ira, Chang Hee Kyung, Kim Hui Taek, Kim Se-Kwon
Department of Orthopaedic Surgery, Pusan National University Hospital, Busan 602-739, Republic of Korea.
Department of Medicine, Graduate School, Pusan National University, Busan 602-739, Republic of Korea.
Int J Biol Macromol. 2014 Jun;67:360-6. doi: 10.1016/j.ijbiomac.2014.03.053. Epub 2014 Apr 3.
Significant development has been achieved with bioceramics and biopolymer scaffolds in the construction of artificial bone. In the present study, we have developed and compared chitosan-micro hydroxyapatite (chitosan-mHA) and chitosan-nano hydroxyapatite (chitosan-nHA) scaffolds as bone graft substitutes. The biocompatibility and cell proliferation of the prepared scaffolds were checked with preosteoblast (MC3T3-E1) cells. Total Volume (TV), bone volume (BV), bone surface (BS), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular separation (Tb.Sp) were found to be higher in chitosan-nHA than chitosan-mHA scaffold. Hence, we suggest that chitosan-nHA scaffold could be a promising biomaterial for bone tissue engineering.
在人工骨构建方面，生物陶瓷和生物聚合物支架已取得显著进展。在本研究中，我们开发并比较了壳聚糖-微羟基磷灰石（壳聚糖-mHA）和壳聚糖-纳米羟基磷灰石（壳聚糖-nHA）支架作为骨移植替代物。用前成骨细胞（MC3T3-E1）检测所制备支架的生物相容性和细胞增殖情况。发现壳聚糖-nHA支架的总体积（TV）、骨体积（BV）、骨表面积（BS）、小梁厚度（Tb.Th）、小梁数量（Tb.N）和小梁间距（Tb.Sp）均高于壳聚糖-mHA支架。因此，我们认为壳聚糖-nHA支架可能是骨组织工程中有前景的生物材料。
