China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2233-2237. doi: 10.1099/ijs.0.061853-0. Epub 2014 Apr 7.
A novel orange-pigmented strain, designated R2-35(T), was isolated from a glacier till near Ny-Alesund, Svalbard Archipelago, Norway. The cells were aerobic, Gram-negative, rod-shaped and sometimes filamentous. Growth occurred at 4-28 °C (optimum, 20 °C), at pH 7.0-9.0 (optimum, pH 8.0) and with 0-1% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-35(T) belonged to the genus Spirosoma with sequence similarity to related species ranging from 91.65 to 95.19%. Strain R2-35(T) contained C16 : 0 (10.7%), C18 : 0 (9.2%), C16 : 1ω5c (16.5%) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (24.6%) as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain R2-35(T) was 54.9 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-35(T) is considered to represent a novel species of the genus Spirosoma, for which the name Spirosoma arcticum sp. nov., is proposed, The type strain is R2-35(T) ( = CCTCC AB 2012849(T) = LMG 28141(T)).
一株新型的橙色着色菌株，命名为 R2-35(T)，是从挪威斯瓦尔巴群岛的新奥尔松附近的冰川土壤中分离出来的。细胞为需氧、革兰氏阴性、杆状，有时呈丝状。在 4-28°C（最佳温度 20°C）、pH 值为 7.0-9.0（最佳 pH 值为 8.0）和 0-1%NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 R2-35(T)属于螺旋体属，与相关种的序列相似性在 91.65%至 95.19%之间。菌株 R2-35(T)含有 C16:0（10.7%）、C18:0（9.2%）、C16:1ω5c（16.5%）和总和特征 3（C16:1ω6c 和/或 C16:1ω7c）（24.6%）作为主要细胞脂肪酸，MK-7 作为主要呼吸醌，磷脂酰乙醇胺作为主要极性脂。菌株 R2-35(T)的 DNA G+C 含量为 54.9 mol%。基于系统发育、生理和化学分类学数据，菌株 R2-35(T)被认为代表了螺旋体属的一个新种，建议命名为北极螺旋体 Spirosoma arcticum sp. nov.，其模式株为 R2-35(T)（ = CCTCC AB 2012849(T) = LMG 28141(T)）。
