3rd Surgery Clinic, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Endocrinology, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Int J Nanomedicine. 2014 Mar 22;9:1453-61. doi: 10.2147/IJN.S54950. eCollection 2014.
Systemic infections caused by methicillin-resistant Staphylococcus aureus (MRSA) and other bacteria are responsible for millions of deaths worldwide, and much of this mortality is due to the rise of antibiotic-resistant organisms as a result of natural selection. Gold nanoparticles synthesized using the standard wet chemical procedure were photoexcited using an 808 nm 2 W laser diode and further administered to MRSA bacteria. Flow cytometry, transmission electron microscopy, contrast phase microscopy, and fluorescence microscopy combined with immunochemical staining were used to examine the interaction of the photoexcited gold nano-particles with MRSA bacteria. We show here that phonon-phonon interactions following laser photoexcitation of gold nanoparticles exhibit increased MRSA necrotic rates at low concentrations and short incubation times compared with MRSA treated with gold nanoparticles alone. These unique data may represent a step forward in the study of bactericidal effects of various nanomaterials, with applications in biology and medicine.
耐甲氧西林金黄色葡萄球菌（MRSA）和其他细菌引起的全身感染导致了全球数百万人的死亡，而这种死亡率的大部分是由于自然选择导致抗生素耐药生物的增加。使用标准湿化学程序合成的金纳米粒子使用 808nm 2W 激光二极管进行光激发，并进一步施用于 MRSA 细菌。流式细胞术、透射电子显微镜、对比相显微镜和荧光显微镜结合免疫化学染色用于检查光激发的金纳米粒子与 MRSA 细菌的相互作用。我们在这里表明，与单独用金纳米粒子处理的 MRSA 相比，激光光激发后金纳米粒子的声子-声子相互作用在低浓度和短孵育时间下表现出增加的 MRSA 坏死率。这些独特的数据可能代表着在研究各种纳米材料的杀菌效果方面向前迈出了一步，在生物学和医学中有应用。
