Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.
Department of Surgery, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
Sci Rep. 2016 Dec 23;6:39466. doi: 10.1038/srep39466.
There are serious systemic infections associated with methicillin-resistant Staphylococcus aureus (MRSA) and several other types of bacteria leading to the deaths of millions of people globally. This type of mortality is generally caused by the increasing number of antibiotic-resistant organisms, a consequence of evolution via natural selection. After the synthesis of gold nanoparticles (GNPs) by wet chemistry, bio-functionalization with IgG molecules was performed. Following administration of IgG-GNPs to MRSA cultures at various concentrations and various incubation time laser irradiation was performed. To assess the selectivity and specificity of the proposed treatment the following methods were used: flow cytometry, contrast phase microscopy, and by fluorescence microscopy. The results in our study indicate that following administration of IgG-GNPs biomolecule an extended and selective bacterial death occurs following laser irradiation in a dose dependent manner. Therefore, the new findings might impel studies on these antibacterial nanomaterials and their biological and medical applications.
耐甲氧西林金黄色葡萄球菌（MRSA）和其他几种细菌会导致全球数百万人死亡，与这些细菌相关的是严重的全身感染。这种死亡率通常是由抗生素耐药生物体的数量增加引起的，这是自然选择进化的结果。通过湿化学法合成金纳米粒子（GNPs）后，用 IgG 分子进行生物功能化。在不同浓度和不同孵育时间下将 IgG-GNPs 施用于 MRSA 培养物后，进行激光照射。为了评估所提出的治疗方法的选择性和特异性，使用了以下方法：流式细胞术、相差显微镜和荧光显微镜。我们的研究结果表明，在给予 IgG-GNPs 生物分子后，在激光照射下会发生延长和选择性的细菌死亡，这是一种剂量依赖性的方式。因此，新的发现可能会推动对这些抗菌纳米材料及其生物和医学应用的研究。
