Kunming Medical University Haiyuan College, Kunming, PR China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat- Sen University, Guangzhou, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3091-3095. doi: 10.1099/ijsem.0.004133.
A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004, was isolated from the tubers of Blume collected from Yunnan Province, PR China. The 16S rRNA gene sequence result showed that the strain SYSUP0004 shared low similarity (97.7 %) with the type strain of . SYSUP0004 grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4 with an interpeptide bridge l-ornithine-d-glutamic acid. Cell-wall sugars were mannose, ribose, glucose, galactose and fucose. The menaquinone was MK-9(H). The major fatty acids were anteiso-C anteiso-C A, C and anteiso-C. The polar lipids of SYSUP0004 were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid. The genomic DNA G+C content was 76.5 %. The average nucleotide identity values between SYSUP0004 and members of the genus were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity. Thus, based on the above results strain SYSUP0004 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain, SYSUP0004 (=KCTC 49025=CGMCC 1.16405).
一株革兰氏阳性、兼性厌氧、非运动的菌株，被命名为 SYSUP0004，是从中国云南省采集的 Blume 块茎中分离得到的。16S rRNA 基因序列结果表明，菌株 SYSUP0004 与 的模式菌株的相似性较低（97.7%）。SYSUP0004 在 pH 6.0-9.0（最佳，pH 8.0）、温度 4-30°C（最佳，28°C）下生长，并能耐受高达 4%的 NaCl（最佳时无 NaCl）。细胞壁肽聚糖类型为 A4，具有 l-鸟氨酸-d-谷氨酸的交叉桥。细胞壁糖为甘露糖、核糖、葡萄糖、半乳糖和岩藻糖。主要的类异戊二烯醌为 MK-9(H)。主要脂肪酸为 anteiso-C anteiso-C A、C 和 anteiso-C。SYSUP0004 的极性脂包括二磷脂酰甘油、未鉴定的磷酸甘油脂、磷脂酰肌醇甘露糖苷和未鉴定的糖脂。基因组 DNA G+C 含量为 76.5%。SYSUP0004 与属内其他成员的平均核苷酸同一性值低于推荐的种间同一性 ANI 标准（95-96%）。因此，基于上述结果，菌株 SYSUP0004 代表了 属的一个新种，建议命名为 sp. nov.。模式菌株 SYSUP0004（=KCTC 49025=CGMCC 1.16405）。
