• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

动力蛋白持续性调控、微管相关蛋白（MAPs）以及微管末端对定位mRNA定向运动的影响。

The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA.

作者信息

Soundararajan Harish Chandra, Bullock Simon L

机构信息

Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.

出版信息

Elife. 2014 Apr 15;3:e01596. doi: 10.7554/eLife.01596.

DOI:10.7554/eLife.01596
PMID:24737859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3985186/
Abstract

Many cellular constituents travel along microtubules in association with multiple copies of motor proteins. How the activity of these motors is regulated during cargo sorting is poorly understood. In this study, we address this issue using a novel in vitro assay for the motility of localising Drosophila mRNAs bound to native dynein-dynactin complexes. High precision tracking reveals that individual RNPs within a population undergo either diffusive, or highly processive, minus end-directed movements along microtubules. RNA localisation signals stimulate the processive movements, with regulation of dynein-dynactin's activity rather than its total copy number per RNP, responsible for this effect. Our data support a novel mechanism for multi-motor translocation based on the regulation of dynein processivity by discrete cargo-associated features. Studying the in vitro responses of RNPs to microtubule-associated proteins (MAPs) and microtubule ends provides insights into how an RNA population could navigate the cytoskeletal network and become anchored at its destination in cells. DOI: http://dx.doi.org/10.7554/eLife.01596.001.

摘要

许多细胞成分与多个拷贝的运动蛋白结合，沿微管移动。在货物分拣过程中，这些运动蛋白的活性是如何被调控的，目前还知之甚少。在本研究中，我们使用一种新型体外分析方法，来研究与天然动力蛋白-动力蛋白激活蛋白复合物结合的定位果蝇mRNA的运动性，以此解决这一问题。高精度追踪显示，群体中的单个核糖核蛋白颗粒（RNP）会沿着微管进行扩散运动或高度持续性的、向负端的移动。RNA定位信号刺激持续性运动，动力蛋白-动力蛋白激活蛋白活性的调控而非每个RNP的总拷贝数，对此效应负责。我们的数据支持一种基于离散货物相关特征对动力蛋白持续性进行调控的新型多运动蛋白易位机制。研究RNP对微管相关蛋白（MAP）和微管末端的体外反应，有助于深入了解RNA群体如何在细胞骨架网络中导航并锚定在其细胞内目的地。DOI: http://dx.doi.org/10.7554/eLife.01596.001

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/564b3bbbba69/elife01596f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/a9c49a5f43f8/elife01596f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/8c32abdca7d7/elife01596fs001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/0087ed3d91c6/elife01596f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/fbc640587cde/elife01596fs002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/17f811cc42d9/elife01596fs003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/d03057899d30/elife01596f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/7e75a5c5f695/elife01596fs004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/6ad39b40ee2d/elife01596fs005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/848459ec9021/elife01596f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/d8754be0e87b/elife01596fs006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/c4d6b6046744/elife01596f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/df627b09d721/elife01596fs007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/a25d2826e0cc/elife01596fs008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/caff600bdecd/elife01596f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/564b3bbbba69/elife01596f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/a9c49a5f43f8/elife01596f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/8c32abdca7d7/elife01596fs001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/0087ed3d91c6/elife01596f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/fbc640587cde/elife01596fs002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/17f811cc42d9/elife01596fs003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/d03057899d30/elife01596f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/7e75a5c5f695/elife01596fs004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/6ad39b40ee2d/elife01596fs005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/848459ec9021/elife01596f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/d8754be0e87b/elife01596fs006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/c4d6b6046744/elife01596f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/df627b09d721/elife01596fs007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/a25d2826e0cc/elife01596fs008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/caff600bdecd/elife01596f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeb7/3985186/564b3bbbba69/elife01596f007.jpg

相似文献

1
The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA.动力蛋白持续性调控、微管相关蛋白（MAPs）以及微管末端对定位mRNA定向运动的影响。
Elife. 2014 Apr 15;3:e01596. doi: 10.7554/eLife.01596.
2
Single-molecule assays reveal that RNA localization signals regulate dynein-dynactin copy number on individual transcript cargoes.单分子分析揭示 RNA 定位信号调节单个转录本货物上的动力蛋白-动力蛋白激活蛋白复合物的拷贝数。
Nat Cell Biol. 2012 Feb 26;14(4):416-23. doi: 10.1038/ncb2446.
3
Dynactin suppresses the retrograde movement of apically localized mRNA in Drosophila blastoderm embryos.动力蛋白激活蛋白抑制果蝇胚盘胚中顶端定位mRNA的逆向运动。
RNA. 2007 Nov;13(11):1860-7. doi: 10.1261/rna.509007. Epub 2007 Sep 27.
4
Dynactin increases the processivity of the cytoplasmic dynein motor.动力蛋白激活蛋白可提高胞质动力蛋白的持续运动能力。
Nat Cell Biol. 2000 Jan;2(1):20-4. doi: 10.1038/71338.
5
RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs.RNA 指导的细胞质动力蛋白-1在重建的转运 RNA 颗粒中的激活。
Elife. 2018 Jun 26;7:e36312. doi: 10.7554/eLife.36312.
6
Processive bidirectional motion of dynein-dynactin complexes in vitro.动力蛋白-动力肌动蛋白复合物在体外的持续双向运动。
Nat Cell Biol. 2006 Jun;8(6):562-70. doi: 10.1038/ncb1421. Epub 2006 May 21.
7
Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.无脑回畸形蛋白-1 促进动力蛋白和动力蛋白激活蛋白复合物向运输 mRNA 的募集。
J Cell Biol. 2013 Aug 5;202(3):479-94. doi: 10.1083/jcb.201211052.
8
Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network Reorganization.动力蛋白-动力蛋白激活蛋白在微管负端的协同积累驱动微管网络重组。
Dev Cell. 2018 Jan 22;44(2):233-247.e4. doi: 10.1016/j.devcel.2017.12.023.
9
Hook Adaptors Induce Unidirectional Processive Motility by Enhancing the Dynein-Dynactin Interaction.钩状适配器通过增强动力蛋白-动力蛋白激活蛋白相互作用诱导单向持续性运动。
J Biol Chem. 2016 Aug 26;291(35):18239-51. doi: 10.1074/jbc.M116.738211. Epub 2016 Jun 30.
10
Dynactin p150 promotes processive motility of DDB complexes by minimizing diffusional behavior of dynein.动力蛋白激活因子 p150 通过最小化动力蛋白的扩散行为来促进 DDB 复合物的进行性运动。
Mol Biol Cell. 2020 Apr 1;31(8):782-792. doi: 10.1091/mbc.E19-09-0495. Epub 2020 Feb 5.

引用本文的文献

1
Synthetic cargo adaptors reveal molecular features that can enhance dynein activation.合成货物衔接蛋白揭示了可增强动力蛋白激活的分子特征。
bioRxiv. 2025 Jun 7:2025.06.06.658359. doi: 10.1101/2025.06.06.658359.
2
Polar localization and local translation of RHO-RELATED PROTEIN FROM PLANTS2 mRNAs promote root hair growth in Arabidopsis.植物中RHO相关蛋白2（RHO-RELATED PROTEIN FROM PLANTS2）mRNA的极性定位和局部翻译促进拟南芥根毛生长。
Plant Cell. 2024 Dec 23;37(1). doi: 10.1093/plcell/koae333.
3
Unraveling the interplay of kinesin-1, tau, and microtubules in neurodegeneration associated with Alzheimer's disease.

本文引用的文献

1
Functions and mechanics of dynein motor proteins.动力蛋白的功能和力学性质。
Nat Rev Mol Cell Biol. 2013 Nov;14(11):713-26. doi: 10.1038/nrm3667. Epub 2013 Sep 25.
2
Cytoplasmic dynein heavy chain: the servant of many masters.细胞质动力蛋白重链：多位主人的仆人。
Trends Neurosci. 2013 Nov;36(11):641-51. doi: 10.1016/j.tins.2013.08.001. Epub 2013 Sep 10.
3
Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.无脑回畸形蛋白-1 促进动力蛋白和动力蛋白激活蛋白复合物向运输 mRNA 的募集。
揭示驱动蛋白-1、tau蛋白和微管在阿尔茨海默病相关神经退行性变中的相互作用。
Front Cell Neurosci. 2024 Oct 23;18:1432002. doi: 10.3389/fncel.2024.1432002. eCollection 2024.
4
Nanoscale dynamics and localization of single endogenous mRNAs in stress granules.应激颗粒中内源性单个 mRNAs 的纳米级动力学和定位
Nucleic Acids Res. 2024 Aug 27;52(15):8675-8686. doi: 10.1093/nar/gkae588.
5
An RNA-based feed-forward mechanism ensures motor switching in oskar mRNA transport.基于 RNA 的前馈机制确保 Oskar mRNA 运输中的运动切换。
J Cell Biol. 2023 Jul 3;222(7). doi: 10.1083/jcb.202301113. Epub 2023 May 22.
6
Antifungal Effect of Nanoparticles against COVID-19 Linked Black Fungus: A Perspective on Biomedical Applications.纳米颗粒对与 COVID-19 相关的黑曲霉的抗真菌作用：对生物医学应用的展望。
Int J Mol Sci. 2022 Oct 19;23(20):12526. doi: 10.3390/ijms232012526.
7
Neurexin and frizzled intercept axonal transport at microtubule minus ends to control synapse formation.神经连接蛋白和卷曲受体在微管负端阻断轴突运输，从而控制突触形成。
Dev Cell. 2022 Aug 8;57(15):1802-1816.e4. doi: 10.1016/j.devcel.2022.06.009. Epub 2022 Jul 8.
8
Microtubule Anchoring: Attaching Dynamic Polymers to Cellular Structures.微管锚定：将动态聚合物附着于细胞结构
Front Cell Dev Biol. 2022 Mar 3;10:867870. doi: 10.3389/fcell.2022.867870. eCollection 2022.
9
Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model.用单速率常数模型区分突触小泡前体对微管末端的导航。
Sci Rep. 2021 Feb 9;11(1):3444. doi: 10.1038/s41598-021-82836-7.
10
RNA-GPS predicts high-resolution RNA subcellular localization and highlights the role of splicing.RNA-GPS 预测高分辨率 RNA 亚细胞定位，并强调剪接的作用。
RNA. 2020 Jul;26(7):851-865. doi: 10.1261/rna.074161.119. Epub 2020 Mar 27.
J Cell Biol. 2013 Aug 5;202(3):479-94. doi: 10.1083/jcb.201211052.
4
Dynein motion switches from diffusive to directed upon cortical anchoring.动力蛋白的运动在皮层锚定后从扩散转变为定向。
Cell. 2013 Jun 20;153(7):1526-36. doi: 10.1016/j.cell.2013.05.020.
5
Molecular adaptations allow dynein to generate large collective forces inside cells.分子适应使动力蛋白能够在细胞内产生大的集体力。
Cell. 2013 Jan 17;152(1-2):172-82. doi: 10.1016/j.cell.2012.11.044.
6
Measuring collective transport by defined numbers of processive and nonprocessive kinesin motors.测量由定义数量的进行性和非进行性驱动蛋白分子所进行的集体运输。
Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):501-6. doi: 10.1073/pnas.1201390110. Epub 2012 Dec 24.
7
Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motors.单分子荧光和激光捕获方法在分子马达研究中的未来挑战。
Dev Cell. 2012 Dec 11;23(6):1084-91. doi: 10.1016/j.devcel.2012.10.002.
8
Reconstitution of the human cytoplasmic dynein complex.人细胞质动力蛋白复合物的重建。
Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20895-900. doi: 10.1073/pnas.1210573110. Epub 2012 Dec 4.
9
Spontaneous motion in hierarchically assembled active matter.层状组装活性物质中的自发运动。
Nature. 2012 Nov 15;491(7424):431-4. doi: 10.1038/nature11591. Epub 2012 Nov 7.
10
In vivo architecture and action of bacterial structural maintenance of chromosome proteins.细菌染色体结构维持蛋白的体内结构和作用。
Science. 2012 Oct 26;338(6106):528-31. doi: 10.1126/science.1227126.