Burke K L, Evans D J, Jenkins O, Meredith J, D'Souza E D, Almond J W
Department of Microbiology, University of Reading, U.K.
J Gen Virol. 1989 Sep;70 ( Pt 9):2475-9. doi: 10.1099/0022-1317-70-9-2475.
A cassette vector has been constructed which allows the rapid and extensive modification of one of the neutralizing antigenic sites of the Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease sites flanking antigenic site 1 have been engineered into a full-length infectious Sabin 1 cDNA clone with minimal alteration to the coding sequence. This facilitates replacement of this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that this region is highly flexible in terms of the number and sequence of amino acids which can be accommodated.
构建了一种盒式载体，可对Sabin 1脊髓灰质炎疫苗株P1/LSc 2ab的一个中和抗原位点进行快速且广泛的修饰。抗原位点1两侧的独特限制性内切酶位点已被设计到全长感染性Sabin 1 cDNA克隆中，对编码序列的改变最小。这便于通过编码外源氨基酸序列的寡核苷酸替换该区域。我们的结果表明，就可容纳的氨基酸数量和序列而言，该区域具有高度灵活性。
