Mari Angela, Napolitano Assunta, Masullo Milena, Pizza Cosimo, Piacente Sonia
Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy.
Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy.
J Pharm Biomed Anal. 2014 Aug 5;96:249-55. doi: 10.1016/j.jpba.2014.04.005. Epub 2014 Apr 13.
Helichrysum italicum is widely used in traditional medicine, in cosmetic, in food and pharmaceutical field. In spite of this, very little is known about the chemical composition of its polar extracts. Therefore this study was addressed to the determination of the metabolite profile of the methanol extract of H. italicum flowers, by using LC-ESI(IT)MSMS. This approach oriented the isolation of 14 compounds, whose structures were unambiguously elucidated by NMR as belonging to flavonoid, phenylpropanoid and acylbenzofuran classes. In addition, one novel drimane sesquiterpene was identified. The quantitative determination of the main compounds occurring in the methanol extract of H. italicum flowers was carried out and their content was compared with that of three selected commercial food supplements based on H. italicum, by using LC-ESI(QqQ)MS. In conclusion the wide occurrence, in high amounts, of quinic acid derivatives in all the analyzed samples was highlighted, showing these compounds as chemical markers of the species for standardization procedures.
意大利蜡菊在传统医学、化妆品、食品和制药领域有着广泛应用。尽管如此，人们对其极性提取物的化学成分知之甚少。因此，本研究旨在通过液相色谱 - 电喷雾电离（离子阱）串联质谱法（LC - ESI(IT)MSMS）测定意大利蜡菊花朵甲醇提取物的代谢物谱。该方法导向了14种化合物的分离，通过核磁共振（NMR）明确阐明了其结构，这些化合物属于黄酮类、苯丙素类和酰基苯并呋喃类。此外，还鉴定出一种新型的杜松烷倍半萜。通过液相色谱 - 电喷雾电离（三重四极杆）串联质谱法（LC - ESI(QqQ)MS）对意大利蜡菊花朵甲醇提取物中主要化合物进行了定量测定，并将其含量与三种选定的基于意大利蜡菊的商业食品补充剂的含量进行了比较。总之，所有分析样品中奎宁酸衍生物大量广泛存在这一情况得到了突出显示，表明这些化合物是该物种标准化程序的化学标志物。
