Kasraei Shahin, Sami Lida, Hendi Sareh, Alikhani Mohammad-Yousef, Rezaei-Soufi Loghman, Khamverdi Zahra
Dental Research Center, Department of Restorative Dentistry, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Oral Medicine, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
Restor Dent Endod. 2014 May;39(2):109-14. doi: 10.5395/rde.2014.39.2.109. Epub 2014 Mar 21.
Recurrent caries was partly ascribed to lack of antibacterial properties in composite resin. Silver and zinc nanoparticles are considered to be broad-spectrum antibacterial agents. The aim of the present study was to evaluate the antibacterial properties of composite resins containing 1% silver and zinc-oxide nanoparticles on Streptococcus mutans and Lactobacillus.
Ninety discoid tablets containing 0%, 1% nano-silver and 1% nano zinc-oxide particles were prepared from flowable composite resin (n = 30). The antibacterial properties of composite resin discs were evaluated by direct contact test. Diluted solutions of Streptococcus mutans (PTCC 1683) and Lactobacillus (PTCC 1643) were prepared. 0.01 mL of each bacterial species was separately placed on the discs. The discs were transferred to liquid culture media and were incubated at 37℃ for 8 hr. 0.01 mL of each solution was cultured on blood agar and the colonies were counted. Data was analyzed with Kruskall-Wallis and Mann-Whitney U tests.
Composites containing nano zinc-oxide particles or silver nanoparticles exhibited higher antibacterial activity against Streptococcus mutans and Lactobacillus compared to the control group (p < 0.05). The effect of zinc-oxide on Streptococcus mutans was significantly higher than that of silver (p < 0.05). There were no significant differences in the antibacterial activity against Lactobacillus between composites containing silver nanoparticles and those containing zinc-oxide nanoparticles.
Composite resins containing silver or zinc-oxide nanoparticles exhibited antibacterial activity against Streptococcus mutans and Lactobacillus.
继发龋部分归因于复合树脂缺乏抗菌性能。银和锌纳米颗粒被认为是广谱抗菌剂。本研究的目的是评估含1%银和氧化锌纳米颗粒的复合树脂对变形链球菌和乳酸杆菌的抗菌性能。
用可流动复合树脂制备含0%、1%纳米银和1%纳米氧化锌颗粒的90个盘状片（n = 30）。通过直接接触试验评估复合树脂盘的抗菌性能。制备变形链球菌（PTCC 1683）和乳酸杆菌（PTCC 1643）的稀释溶液。将每种细菌的0.01 mL分别置于盘上。将盘转移至液体培养基中，于37℃孵育8小时。将每种溶液的0.01 mL接种于血琼脂上并计数菌落。数据采用Kruskal-Wallis和Mann-Whitney U检验进行分析。
与对照组相比，含纳米氧化锌颗粒或银纳米颗粒的复合材料对变形链球菌和乳酸杆菌表现出更高的抗菌活性（p < 0.05）。氧化锌对变形链球菌的作用显著高于银（p < 0.05）。含银纳米颗粒的复合材料与含氧化锌纳米颗粒的复合材料对乳酸杆菌的抗菌活性无显著差异。
含银或氧化锌纳米颗粒的复合树脂对变形链球菌和乳酸杆菌表现出抗菌活性。
