Department of Orthopedic Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangdong Province, Guangzhou, China; Department of Orthopedic, the First Hospital of Putian City, Fujian Province, Putian City, China.
Department of Orthopedic Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangdong Province, Guangzhou, China.
PLoS One. 2014 May 5;9(5):e96181. doi: 10.1371/journal.pone.0096181. eCollection 2014.
Alendronate (ALE) is a conventional drug used to treat osteoporosis. Low-magnitude whole-body vibration (WBV) exercise has been developed as a potential treatment for osteoporosis. The aim of this study was to investigate whether low-magnitude WBV could enhance the protective effect of ALE on bone properties in ovariectomized rats.
A total of 128 Sprague-Dawley rats were randomly divided into five groups (SHAM, OVX+VEH, OVX+WBV, OVX + ALE, OVX+WBV+ALE). The level of WBV applied was 0.3 g at 45-55 Hz for 20 min/day, 5 day/week and for 3 months. ALE was administered in dose of 1 mg/Kg once a week. Every four weeks eight rats from each group were sacrificed and their blood and both tibiae were harvested. The expression of osteocalcin and CTX in serum was measured by enzyme-linked immunosorbent assay (ELISA) and the tibiae were subjected to metaphyseal three-point bending and μCT analysis.
Osteocalcin rose after ovariectomy and was not appreciably changed by either alendronate or WBV alone or in combination. Alendronate treatment significantly prevented an increase in CTX. WBV alone treatment did not alter this effect. Compared with the OVX+WBV group, nearly all tested indices such as the BV/TV, TV apparent, Tb.N, Tb.Th, and Conn.D were higher in the OVX+ALE group at week 12.Compared with the OVX+WBV group, certain tested indices such as BV/TV, TV apparent, Tb.N, and Con.D, were higher in the OVX+WBV+ALE group at week 12. At week 12, tibiae treated with WBV+ALE exhibited a significantly higher Fmax compared to the OVX+VEH group, and a significant difference was also found in energy absorption between the OVX+WBV+ALE and OVX+VEH groups.
Compared with the WBV, ALE was more effective at preventing bone loss and improved the trabecular architecture. However, WBV enhanced the effect of alendronate in ovariectomized rats by inducing further improvements in trabecular architecture.
阿仑膦酸钠（ALE）是一种用于治疗骨质疏松症的常规药物。低强度全身振动（WBV）运动已被开发为治疗骨质疏松症的一种潜在方法。本研究旨在探讨低强度 WBV 是否可以增强 ALE 对去卵巢大鼠骨特性的保护作用。
共 128 只 Sprague-Dawley 大鼠随机分为五组（SHAM、OVX+VEH、OVX+WBV、OVX+ALE、OVX+WBV+ALE）。应用的 WBV 强度为 0.3g，频率为 45-55Hz，每天 20 分钟，每周 5 天，共 3 个月。每周给予 ALE 剂量为 1mg/Kg。每四周每组处死 8 只大鼠，采集其血清和双侧胫骨。通过酶联免疫吸附试验（ELISA）测量血清中骨钙素和 CTX 的表达，对胫骨进行骺板三点弯曲和μCT 分析。
去卵巢后骨钙素升高，单独使用阿仑膦酸钠或 WBV 或两者联合使用均未明显改变。阿仑膦酸钠治疗可显著预防 CTX 增加。单独使用 WBV 治疗并未改变这种作用。与 OVX+WBV 组相比，在第 12 周时，几乎所有测试指标，如 BV/TV、TV 表观、Tb.N、Tb.Th 和 Conn.D，在 OVX+ALE 组中均较高。与 OVX+WBV 组相比，在第 12 周时，在 OVX+WBV+ALE 组中，某些测试指标，如 BV/TV、TV 表观、Tb.N 和 Con.D，较高。在第 12 周时，用 WBV+ALE 处理的胫骨的 Fmax 与 OVX+VEH 组相比显著升高，而在 OVX+WBV+ALE 组与 OVX+VEH 组之间，能量吸收也存在显著差异。
与 WBV 相比，ALE 更能有效预防骨丢失并改善小梁结构。然而，WBV 通过进一步改善小梁结构增强了 ALE 对去卵巢大鼠的作用。
