血液分析仪中的技术与新的荧光流式细胞术参数
Technology and new fluorescence flow cytometry parameters in hematological analyzers.
作者信息
Arneth Borros M, Menschikowki Mario
机构信息
Institute of Clinical Chemistry and Laboratory Medicine, Carl Gustav Carus University Hospital, University of Technology Dresden, Dresden, Germany.
出版信息
J Clin Lab Anal. 2015 May;29(3):175-83. doi: 10.1002/jcla.21747. Epub 2014 May 5.
BACKGROUND
Modern hematological analyzers offer a series of new tests in addition to the differential blood cell count. Here, we provide a short overview of the technique and the new tests included in the hematological analyzers manufactured by the company Sysmex (Kobe, Japan). As introduced here, the resulting new extended blood cell count allows for the quantification of DNA- and RNA-rich, activated and/or precursor leukocyte subpopulations.
METHODS
To compare the different methods of blood cell counting, repetitive cell counting is carried out by hematological experts using microscopy and using the XE-5000 hematological analyzer.
RESULTS
The hematological analyzer delivers more-accurate results with respect to normal cells but microscopy is suited better to recognize and count pathological cells.
CONCLUSION
With the new hematological analyzers, several new hematological tests are made available as screening tools that were not possible previously using standard cell-counting procedures.
背景
现代血液分析仪除了进行血细胞分类计数外，还提供一系列新的检测项目。在此，我们简要概述由日本神户Sysmex公司生产的血液分析仪所采用的技术及包含的新检测项目。如本文所述，由此产生的新扩展血细胞计数能够对富含DNA和RNA、活化的和/或前体白细胞亚群进行定量分析。
方法
为比较不同的血细胞计数方法，血液学专家使用显微镜和XE - 5000血液分析仪进行重复细胞计数。
结果
血液分析仪在正常细胞计数方面能提供更准确的结果，但显微镜更适合识别和计数病理细胞。
结论
借助新型血液分析仪，一些新的血液学检测项目可作为筛查工具使用，而这在以前使用标准细胞计数程序时是无法实现的。
Zotero 插件