Tao Zexin, Liu Guifang, Wang Min, Wang Huanyu, Lin Xiaojuan, Song Lizhi, Wang Suting, Wang Haiyan, Liu Xiaodong, Cui Ning, Song Yanyan, Xu Aiqiang
1] Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China [2] Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China [3].
School of Public Health, Shandong University, Jinan, People's Republic of China.
Sci Rep. 2014 May 9;4:4908. doi: 10.1038/srep04908.
Japanese encephalitis virus (JEV) can cause serious encephalitis and Culex mosquitoes are the primary vector. In 2013, a JE outbreak occurred in Shandong Province, China with 407 confirmed cases, including 11 deaths. An investigation on JEV in mosquitoes during the outbreak was conducted. A total of 14,719 mosquitoes were collected at 3 sites. For the 12,695 Culex tritaeniorhynchus mosquitoes, 88/201 pooled samples were positive by RT-PCR for the presence of the pre-membrane or envelope protein coding genes. The maximum likelihood estimates of JEV positive individuals per 1,000 vectors were 12.0, 7.2, and 6.0 in the 3 sites respectively with an overall estimate of 9.1. Phylogenetic analysis on these pre-membrane (n = 72) and envelope (n = 26) sequences with those of reference strains revealed they belonged to genotype I. This study describes the molecular epidemiology of JEV and suggests the high infection rate in mosquitoes is an important factor for the outbreak.
日本脑炎病毒（JEV）可引发严重脑炎，库蚊是主要传播媒介。2013年，中国山东省发生了一次日本脑炎疫情，确诊病例达407例，其中11人死亡。疫情期间对蚊子中的JEV进行了调查。在3个地点共采集了14,719只蚊子。对于12,695只三带喙库蚊，88/201份混合样本经逆转录聚合酶链反应（RT-PCR）检测，其前膜或包膜蛋白编码基因呈阳性。每1000只媒介中JEV阳性个体的最大似然估计值在3个地点分别为12.0、7.2和6.0，总体估计值为9.1。对这些前膜序列（n = 72）和包膜序列（n = 26）与参考毒株进行系统发育分析，结果显示它们属于基因型I。本研究描述了JEV的分子流行病学情况，并表明蚊子的高感染率是此次疫情爆发的一个重要因素。
