College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Int J Biol Macromol. 2014 Aug;69:146-50. doi: 10.1016/j.ijbiomac.2014.05.004. Epub 2014 May 10.
This study compared the anti-inflammatory activities of APS and SAPS in LPS-treated broiler chicks. The sulfated modification of these compounds was performed using the classic chlorosulfonic acid-pyridine method. On d 16, the birds were injected intramuscularly with 0.5mL of either saline, APS (4 or 8mg/kg BW) or SAPS (4 or 8mg/kg BW) once a day for three successive days. On days 19 and 20, the birds were intraperitoneally injected with 0.5mL of LPS (1mg/kg BW). Saline was used as the blank control. The results showed that the LPS-treated birds exhibited higher expression of the pro-inflammatory cytokines IL-1β and IFN-γ and lower expression of the tight junction proteins ZO-2 and occludin in the jejunum. Administration of SAPS down-regulated the expression of jejunal TNF-α and IL-1β. In addition, the expression of both ZO-2 and occludin was higher in birds that received high doses of APS and SAPS. On the other hand, APS and SAPS had no effect on the condition of the immune system. The expression of TLR4 in the jejunum was lower in the low-dose SAPS group. Our findings suggest that SAPS is a more effective anti-inflammatory agent than APS in vivo.
本研究比较了 APS 和 SAPS 在 LPS 处理的肉鸡中的抗炎活性。这些化合物的硫酸化修饰是使用经典的氯磺酸-吡啶法进行的。在第 16 天，鸡肌肉注射 0.5mL 生理盐水、APS（4 或 8mg/kgBW）或 SAPS（4 或 8mg/kgBW），连续 3 天每天一次。在第 19 和 20 天，鸡腹膜内注射 0.5mL LPS（1mg/kgBW）。生理盐水作为空白对照。结果表明，LPS 处理的鸡在空肠中表现出更高的促炎细胞因子 IL-1β 和 IFN-γ 的表达，以及更低的紧密连接蛋白 ZO-2 和 occludin 的表达。SAPS 给药下调了空肠 TNF-α 和 IL-1β 的表达。此外，高剂量 APS 和 SAPS 组的 ZO-2 和 occludin 的表达更高。另一方面，APS 和 SAPS 对免疫系统没有影响。低剂量 SAPS 组空肠 TLR4 的表达降低。我们的研究结果表明，SAPS 是一种比 APS 更有效的体内抗炎剂。
